Matsushita Makoto, Otani Kana, Sakamoto Yui, Arai Tomoko, Yukimasa Nobuyasu, Muramoto Yoshimi, Komoda Tsugikazu
Rinsho Byori. 2015 May;63(5):543-7.
Intestinal alkaline phosphatase (IAP) appears in the circulation more frequently in blood group B or O secretors than in blood group A or AB secretors and non-secretors, and serum IAP activity rises following the ingestion of a high-fat meal. In a previous study, the occurrence of two IAP isoforms, with high (HIAP) and normal molecular mass (NIAP), in healthy sera was demonstrated by 6.0% polyacrylamide gel electrophoresis in the presence of 1% Triton X-100. NIAP was present in the fasting serum of only healthy blood group B or O secretors, but was present in all subjects following ingestion of a high-fat meal. We classified 56 healthy subjects into 3 blood groups: B (n = 19), O (n = 17), and A (n = 20), and measured their serum ALP activity in a fasting state and 6 h after a high-fat meal. The amount of ABH substances in the saliva of each subject was determined by the hemagglutination inhibition test. Correlation coefficients between the change in ALP activity after high-fat meal ingestion and the hemagglutination inhibition values in saliva were 0.925 in blood group B, 0.879 in blood group O, and 0.906 in blood group A. These results suggest that increases in ALP activity in the circulation following the ingestion of a high-fat meal are closely related to the amount of ABH substances in saliva.
肠道碱性磷酸酶（IAP）在B型或O型血型分泌者的血液循环中出现的频率高于A型或AB型血型分泌者及非分泌者，并且在摄入高脂餐后血清IAP活性会升高。在先前的一项研究中，通过在含有1% Triton X - 100的条件下进行6.0%聚丙烯酰胺凝胶电泳，证实在健康血清中存在两种IAP同工型，即高分子质量（HIAP）和正常分子质量（NIAP）的同工型。NIAP仅存在于健康B型或O型血型分泌者的空腹血清中，但在所有受试者摄入高脂餐后均存在。我们将56名健康受试者分为3个血型组：B型（n = 19）、O型（n = 17）和A型（n = 20），并测量了他们在空腹状态和高脂餐后6小时的血清碱性磷酸酶（ALP）活性。通过血凝抑制试验测定了每个受试者唾液中ABH物质的量。高脂餐摄入后ALP活性变化与唾液中血凝抑制值之间的相关系数在B型血中为0.925，在O型血中为0.879，在A型血中为0.906。这些结果表明，高脂餐摄入后循环中ALP活性的增加与唾液中ABH物质的量密切相关。
