Shimizu Tomomi, Imanishi Akio, Sugimoto Kenichi, Takeda Nobuaki, Hirata Ryuuzou, Andou Takashi, Morikawa Seiichi, Suzuki Yoshio, Watanabe Masayuki, Okuta Miho, Kawana Takayuki, Namikawa Yui, Suzuki Masato, Watanabe Makino, Okada Takao, Ohta Makoto
Corresponding author: Tomomi SHIMIZU, Department of Biomedical Engineering, Toin University of Yokohama, Yokohama, Kanagawa 225-8502, Japan.
Rinsho Byori. 2011 Oct;59(10):930-5.
Running for an extended period of time can cause severe stress on the body, subsequently damaging skeletal muscle and resulting in changes in blood components. However, few reports have examined vital responses during and after running. This study analyzed inflammatory responses during and after running and changes in stress responses as determined by serial changes in blood components. Venous blood was obtained before starting, 6 h after starting, 12 h after starting, and immediately after finishing 24 h of continuous running. Samples were analyzed for high-sensitivity C-reactive protein (hsCRP), pentraxin 3 (ptx3), white blood cells (WBC), myoglobin, creatine kinase (CK), and hormones. Diet and physical activity were standardized 24 h before and after running. Subjects comprised 16 men who agreed to participate in experimental running on November 8 and 9, 2008, at Tokyo Gakugei University. Mean running distance was 151.32 +/- 32.1 km (range, 83.6-210.0 km) in 24 h. A significant increase in hsCRP was seen from 12 h after starting to completion. Compared to hsCRP, ptx3 gradually increased from before starting to after completion, showing a significant difference between pre and post-run ptx3 levels. WBC count increased significantly until 6 h after starting. Neutrophils in leukocytosis increased significantly during the first 6 h. Eosinophils decreased significantly over the course of the 24 h. Cortisol increased, and testosterone decreased significantly from 6 h after starting. Dehydroepiandrosterone sulfate (DHEA-S), myoglobin, and CK increased over the course of the 24 h. Reactive oxygen metabolites (d-ROMs) changed within the normal range though there was a significant decrease, and biological anti-oxidant potential (BAP) stabilized. Active natural killer cells decreased significantly after 24 h running. Biopyrrin (BPn) increased significantly. Changes in stress oxide were small both during and after running, and adaptation for antioxidation was good. DHEAS, a biomarker of aging, was found to increase over the course of the 24 h, suggesting that controlling decreases in DHEA-S may be possible using exercise, particularly in males. The key finding was that DHEA S levels tended to increase with continuous aerobic exercise.
长时间跑步会给身体带来严重压力，进而损害骨骼肌并导致血液成分发生变化。然而，很少有报告研究跑步期间及之后的重要反应。本研究分析了跑步期间及之后的炎症反应以及由血液成分的连续变化所确定的应激反应变化。在开始跑步前、开始后6小时、开始后12小时以及连续跑步24小时结束后立即采集静脉血。对样本进行高敏C反应蛋白（hsCRP）、五聚素3（ptx3）、白细胞（WBC）、肌红蛋白、肌酸激酶（CK）和激素分析。跑步前后24小时的饮食和身体活动标准化。受试者包括16名男性，他们于2008年11月8日和9日在东京教育大学同意参与实验性跑步。24小时内平均跑步距离为151.32±32.1公里（范围83.6 - 210.0公里）。从开始后12小时到结束，hsCRP显著增加。与hsCRP相比，ptx3从开始前到结束后逐渐增加，跑步前后ptx3水平存在显著差异。白细胞计数在开始后6小时内显著增加。白细胞增多症中的中性粒细胞在最初6小时内显著增加。嗜酸性粒细胞在24小时内显著减少。皮质醇增加，睾酮从开始后6小时起显著下降。硫酸脱氢表雄酮（DHEA - S）、肌红蛋白和CK在24小时内增加。活性氧代谢产物（d - ROMs）虽有显著下降但在正常范围内变化，生物抗氧化潜能（BAP）稳定。24小时跑步后活性自然杀伤细胞显著减少。生物吡咯（BPn）显著增加。跑步期间及之后应激氧化物的变化较小，抗氧化适应良好。衰老生物标志物DHEAS在24小时内增加，这表明通过运动控制DHEA - S的减少可能是可行的，尤其是在男性中。关键发现是DHEA S水平倾向于随着持续有氧运动而增加。
