Reśliński Adrian, Mikucka Agnieszka, Kwiecińska-Piróg Joanna, Głowacka Katarzyna, Gospodarek Eugenia, Dabrowiecki Stanisław
Katedra i Klinika Chirurgii Ogólnej i Endokrynologicznej Collegium Medicum im. Ludwika Rydygiera w Bydgoszczy Uniwersytetu Mikołaja Kopernika w Toruniu.
Med Dosw Mikrobiol. 2011;63(1):21-7.
A serious complication of hernioplasty with the use of a biomaterial implant is deep surgical site infection (SSI) encompassing the implant. Among the most common etiological factors of deep SSI in patients after hernioplasty are Staphylococcus aureus and Escherichia coli strains, which may create a biofilm on the surface of synthetic implants. The aim of this study was assessment of biofilm formation by S. aureus and E. coli on the surface ofpolypropylene mesh. The study included 108 strains (62 S. aureus and 46 E. coli) from the collection of Department of Microbiology Collegium Medicum im. L. Rydygier in Bydgoszcz, Nicolaus Copernicus University in Torun (CM UMK). Evaluation of biofilm formation was performed using the method of reduction of 2,3,5-triphenyltetrazolium chloride (TTC) and a scanning electron microscope. In the group of S. aureus strains, 88.7% isolates formed biofilm very strongly, 1.6% strongly, and 9.7% poor. Among E. coli strains, 54.3% isolates were characterized by very strong biofilm formation, while 45.7% strong biofilm formation. Strains ofS. aureus strongly than E. coli form a biofilm on the surface of monofilament polypropylene mesh.
使用生物材料植入物进行疝修补术的一个严重并发症是包括植入物在内的深部手术部位感染（SSI）。疝修补术后患者深部SSI最常见的病因是金黄色葡萄球菌和大肠杆菌菌株，它们可能在合成植入物表面形成生物膜。本研究的目的是评估金黄色葡萄球菌和大肠杆菌在聚丙烯网片表面形成生物膜的情况。该研究纳入了来自托伦哥白尼大学比得哥什医学院微生物学系（CM UMK）的108株菌株（62株金黄色葡萄球菌和46株大肠杆菌）。使用氯化三苯基四氮唑（TTC）还原法和扫描电子显微镜对生物膜形成进行评估。在金黄色葡萄球菌菌株组中，88.7%的分离株形成非常强的生物膜，1.6%形成强生物膜，9.7%形成弱生物膜。在大肠杆菌菌株中，54.3%的分离株表现为非常强的生物膜形成，而45.7%形成强生物膜。金黄色葡萄球菌菌株比大肠杆菌菌株在单丝聚丙烯网片表面形成生物膜的能力更强。
