Kwiecińska-Piróg Joanna, Bogiel Tomasz, Gospodarek Eugenia
Katedra i Zakład Mikrobiologii, Collegium Medicum im. L. Rydygiera w Bydgoszczy, Uniwersytet Mikołaja Kopernika w Toruniu.
Med Dosw Mikrobiol. 2011;63(2):131-8.
Proteus sp. rods are opportunistic human pathogens. These microorganisms are mainly isolated from patients with urinary tract infections, particularly associated with using of biomaterials, on which surface they can form biofilm. The aim of our study was the estimation of Proteus mirabilis rods ability to form biofilm on the surface of 5 biomaterials (polychloride vinyl, silicone latex, polypropylene, polybutylen teraftalan and polyamide) using Richards' and quantitative method and comparison results of both methods. A total number of 84 P. mirabilis strains were included into the study. All of them were isolated in the Department of Clinical Microbiology University Hospital no. 1 of dr A. Jurasz Collegium Medicum of L. Rydygier in Bydgoszcz, Nicolaus Copernicus University in Toruń between 2005 and 2008. Examined P. mirabilis strains formed heavy biofilm with statistically significantly values on the surface of silicone latex than on polychloride vinyl and on polypropylene surface than polybutylen teraftalen or polyamide. High correlation of both methods was established. The Richards' method can be used to quick identification of P. mirabilis biofilm.
变形杆菌属杆菌是人类机会致病菌。这些微生物主要从尿路感染患者中分离得到，尤其与生物材料的使用有关，它们可在生物材料表面形成生物膜。我们研究的目的是使用理查兹法和定量法评估奇异变形杆菌在5种生物材料（聚氯乙烯、硅乳胶、聚丙烯、聚丁烯对苯二甲酸酯和聚酰胺）表面形成生物膜的能力，并比较两种方法的结果。共有84株奇异变形杆菌菌株纳入研究。所有菌株均于2005年至2008年期间在比得哥什的A. 尤拉什医学院第一大学医院临床微生物科、托伦的哥白尼大学分离得到。所检测的奇异变形杆菌菌株在硅乳胶表面形成的生物膜比在聚氯乙烯表面形成的厚，在聚丙烯表面形成的生物膜比在聚丁烯对苯二甲酸酯或聚酰胺表面形成的厚，差异具有统计学意义。两种方法具有高度相关性。理查兹法可用于快速鉴定奇异变形杆菌生物膜。
