Rastawicki Waldemar, Rokosz Natalia, Jagielski Marek
Zakład Bakteriologii Narodowego Instytutu Zdrowia Publicznego-Państwowego Zakladu Higieny, Warszawie.
Med Dosw Mikrobiol. 2011;63(1):65-71.
Salmonella Typhimurium and Salmonella Enteritidis are the two predominant serogroups, responsible for about 80% of all human cases of salmonelosis in Poland. Therefore we compared the usefulness of lipopolysaccharides antigens extracted by phenol (Westphal method) and trichloroacetic acid (Boivine method) from Salmonella Typhimurium and Enteritidis in ELISA method for the determination of antibodies. We used one home - made LPS antigen and two others commercially available antigens from SIGMA - Aldrich. Our study showed that the presence of antibodies was found in 35 (74.5%) sera from 47 samples from patients with suspected salmonelosis. There was no significant statistical differences of frequency of appearance of antibodies to all three Salmonella antigens in sera from patients with salmonelosis and in sera from control group. This study showed that all three antigens are useful for determination of IgA, IgG, IgM antibodies for Salmonella serogroup B and D in routine serological diagnosis of salmonelosis. However, it should be considered possibility of cross-reaction between LPS antigen of Salmonella and antibodies to Yersinia enterocolitica which could be correlated with similarity between somatic antigens of these two pathogens.
鼠伤寒沙门氏菌和肠炎沙门氏菌是两个主要血清群，在波兰约占所有人类沙门氏菌病病例的80%。因此，我们比较了用苯酚（韦斯特法尔法）和三氯乙酸（博伊文法）从鼠伤寒沙门氏菌和肠炎沙门氏菌中提取的脂多糖抗原在ELISA法中用于测定抗体的效用。我们使用了一种自制的LPS抗原和另外两种购自西格玛奥德里奇公司的市售抗原。我们的研究表明，在47例疑似沙门氏菌病患者的样本中，35份血清（74.5%）中发现了抗体。沙门氏菌病患者血清和对照组血清中针对所有三种沙门氏菌抗原的抗体出现频率没有显著统计学差异。这项研究表明，在沙门氏菌病的常规血清学诊断中，所有三种抗原都可用于测定B群和D群沙门氏菌的IgA、IgG、IgM抗体。然而，应考虑沙门氏菌LPS抗原与小肠结肠炎耶尔森氏菌抗体之间发生交叉反应的可能性，这可能与这两种病原体的菌体抗原相似性有关。
