Karlsson K, Carlsson H E, Neringer R, Lindberg A A
Scand J Infect Dis. 1980;12(1):41-7. doi: 10.3109/inf.1980.12.issue-1.08.
An enzyme immunoassay for serogroup specific immunological diagnosis of salmonella infections has been developed. Antigens representing salmonella O-antigens 4 (serogroup B) and 9 (serogroup D) were prepared by periodate oxidation of phenol-water extracted lipopolysaccharides from S. typhimurium and S. enteritidis respectively. Applied for seroepidemiological studies of S. typhimurium infections such chemically modified antigens showed a superior specificity compared to native lipopolysaccharides. The antibody titres against O-antigen 4 measured by the enzyme-linked immunosorbent assay (ELISA) were detectable during the second week and increased during the first 3--4 weeks of S. typhimurium infection. High titres persisted for at least 2--3 months and in some cases for more than 3 years. For reliable serodiagnosis a significant increase of the titre between acute and convalescent samples is necessary . Determination of ratio between IgG and IgM antibody titres against the pathogen may, however, be used on single serum samples to indicate whether the observed antibody titre is of recent or longstanding origin.
已开发出一种用于沙门氏菌感染血清群特异性免疫诊断的酶免疫测定法。分别通过高碘酸盐氧化从鼠伤寒沙门氏菌和肠炎沙门氏菌中酚水提取的脂多糖，制备了代表沙门氏菌O抗原4（血清群B）和9（血清群D）的抗原。应用于鼠伤寒沙门氏菌感染的血清流行病学研究时，这种化学修饰的抗原与天然脂多糖相比显示出更高的特异性。通过酶联免疫吸附测定（ELISA）测量的针对O抗原4的抗体滴度在感染的第二周可检测到，并在鼠伤寒沙门氏菌感染的前3 - 4周内升高。高滴度至少持续2 - 3个月，在某些情况下持续超过3年。为了进行可靠的血清学诊断，急性期和恢复期样本之间的滴度必须有显著增加。然而，测定针对病原体的IgG和IgM抗体滴度之间的比值可用于单个血清样本，以表明观察到的抗体滴度是近期产生的还是长期存在的。
