Department of Microbiology, School of Life Science, Karpagam University, Tamil Nadu, India.
Pol J Microbiol. 2011;60(3):213-21.
Marine actinomycetes were isolated from sediment samples collected from Pitchavaram mangrove ecosystem situated along the southeast coast of India. Maximum actinomycete population was noted in rhizosphere region. About 38% of the isolates produced L-asparaginase. One potential strain KUA106 produced higher level of enzyme using tryptone glucose yeast extract medium. Based on the studied phenotypic characteristics, strain KUA106 was identified as Streptomyces parvulus KUA106. The optimization method that combines the Plackett-Burman design, a factorial design and the response surface method, which were used to optimize the medium for the production of L-asparaginase by Streptomycetes parvulus. Four medium factors were screened from eleven medium factors by Plackett-Burman design experiments and subsequent optimization process to find out the optimum values of the selected parameters using central composite design was performed. Asparagine, tryptone, d) extrose and NaCl components were found to be the best medium for the L-asparaginase production. The combined optimization method described here is the effective method for screening medium factors as well as determining their optimum level for the production of L-asparaginase by Streptomycetes parvulus KUAP106.
从印度东南沿海皮查瓦拉姆红树林生态系统采集的沉积物样本中分离出海洋放线菌。根际区域的放线菌种群最多。约 38%的分离株产生 L-天冬酰胺酶。一种潜在的菌株 KUA106 在胰蛋白胨葡萄糖酵母提取物培养基中产生了更高水平的酶。根据所研究的表型特征，菌株 KUA106 被鉴定为小链霉菌 KUA106。优化方法结合了 Plackett-Burman 设计、析因设计和响应面法，用于优化小链霉菌生产 L-天冬酰胺酶的培养基。通过 Plackett-Burman 设计实验和后续优化过程，从 11 种培养基因素中筛选出 4 种培养基因素，使用中心组合设计确定所选参数的最佳值。发现天门冬酰胺、胰蛋白胨、d)葡萄糖和 NaCl 成分是 L-天冬酰胺酶生产的最佳培养基。这里描述的组合优化方法是筛选培养基因素以及确定小链霉菌 KUAP106 生产 L-天冬酰胺酶的最佳水平的有效方法。
