Department of Virology, National Institute of Public Heath, National Institute of Hygiene, Warsaw, Poland.
Pol J Microbiol. 2011;60(3):253-8.
Respiratory Syncytial Virus (RSV) is one of the most common causes of lower respiratory tract infections in young children, immunocompromised patients (children and adults), patients with chronic respiratory diseases and elderly people. Reinfections occur throughout the life, but the severity of disease decreased with subsequent infection. The aim of this study was to analyze the frequency of RSV infections in two selected subpopulations: young children (below 5 y.) and adults with chronic respiratory diseases (25-87 y.). Nasopharyngeal swabs (334) collected from October 2008 to March 2010 were examined. The presence of RSV genome was determined by RT-PCR and the presence of RSV antigen by quick immunochromatographic test. Positive results of RT-PCR were found in 45.2% of all swabs: 48.6% samples in 2008; 41.5% in 2009; 50.8% in 2010. The highest frequency of RSV-positive samples was in fall-winter months, but differences in RSV epidemic seasons were found. In the first season (2008-2009) an increased number of RSV infections was observed from November 2008, but in the second season--from January 2010. Generally, the frequency of RSV-positive RT-PCR among children was 53%, among adults 25%. The highest difference was observed in the first three-month period of 2010. RT-PCR positive samples were found in 68.5% of children and 5.9% of adults. However, the RSV antigen was found in 44.4% of samples collected from adults in this period. Our results indicate that the contribution of RSV infections during epidemic season of respiratory tract infections in Poland was really high among children and adults.
呼吸道合胞病毒（RSV）是导致婴幼儿、免疫功能低下者（儿童和成人）、慢性呼吸道疾病患者和老年人下呼吸道感染的最常见原因之一。再感染可发生于整个生命过程中，但随着后续感染，疾病严重程度会降低。本研究旨在分析两种选定亚人群（<5 岁的儿童和 25-87 岁的慢性呼吸道疾病成人）中 RSV 感染的频率。收集了 2008 年 10 月至 2010 年 3 月的鼻咽拭子（334 个）。通过 RT-PCR 检测 RSV 基因组的存在，通过快速免疫层析试验检测 RSV 抗原的存在。所有拭子的 RT-PCR 阳性结果为 45.2%：2008 年 48.6%；2009 年 41.5%；2010 年 50.8%。呼吸道合胞病毒阳性样本的最高频率出现在秋冬季节，但呼吸道合胞病毒流行季节存在差异。在第一个季节（2008-2009 年），2008 年 11 月观察到 RSV 感染数量增加，但在第二个季节（2009-2010 年），2010 年 1 月观察到 RSV 感染数量增加。总体而言，儿童 RSV-PCR 阳性率为 53%，成人 25%。2010 年的前三个月差异最大。RT-PCR 阳性样本在儿童中占 68.5%，在成人中占 5.9%。然而，在此期间，从成人采集的样本中发现了 44.4%的 RSV 抗原。我们的结果表明，在波兰呼吸道感染流行季节，RSV 感染在儿童和成人中的贡献确实很高。
