Bai-Baie Saringat Bin, Laila Lia
Faculty of Pharmacy, Andalas University, Padang, Indonesia.
Pak J Pharm Sci. 2012 Jan;25(1):1-6.
Haruan (Channa striatus) extract was formulated to aerosol for wound and burn treatment. Haruan extract is containing amino acids and fatty acids that important for wound healing process. The purpose of this study is to observe the effect of formulation and other excipients in the formula to amino acids and fatty acids content in Haruan extract before and after formulated into aerosol. Precolumn derivatization with 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate (AQC) method is used for amino acids analysis. Fatty acids in Haruan extract were esterified using transesterification method to form FAMEs before analyzed using GC. Boron trifluoride-methanol reagent is used for transesterification. Tyrosine and methionine concentrations were different after formulated. The concentrations were decrease. There are six fatty acids have amount that significantly different after formulated into concentrate and aerosol. Contents of these fatty acids were increase. Generally, fatty acids which had content increased after formulated were the long-chain fatty acids. This might be happen because of chain extension process. Saponification and decarboxylation would give the chain extended product. Therefore contents of long-chain fatty acids were increase. Generally, the aerosol formulation did not affect the amino acids concentrations in Haruan extract while some long-chain fatty acids concentrations were increase after formulated into concentrate and aerosol.
将哈鲁安（条纹鲶鱼）提取物制成气雾剂用于伤口和烧伤治疗。哈鲁安提取物含有对伤口愈合过程很重要的氨基酸和脂肪酸。本研究的目的是观察制剂配方及其他辅料对哈鲁安提取物制成气雾剂前后氨基酸和脂肪酸含量的影响。采用6-氨基喹啉-N-羟基琥珀酰亚胺基甲酸酯（AQC）方法进行柱前衍生化用于氨基酸分析。哈鲁安提取物中的脂肪酸通过酯交换法进行酯化形成脂肪酸甲酯，然后用气相色谱法进行分析。使用三氟化硼-甲醇试剂进行酯交换。制成气雾剂后酪氨酸和蛋氨酸浓度有所不同，浓度降低。有六种脂肪酸在制成浓缩液和气雾剂后含量有显著差异，这些脂肪酸的含量增加。一般来说，制成气雾剂后含量增加的脂肪酸是长链脂肪酸。这可能是由于链延长过程导致的。皂化和脱羧会产生链延长产物。因此长链脂肪酸的含量增加。一般来说，气雾剂配方不影响哈鲁安提取物中的氨基酸浓度，而一些长链脂肪酸在制成浓缩液和气雾剂后浓度增加。
