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海蛇中的脂肪酸和氨基酸组成在伤口愈合中可能发挥的作用。

Fatty acid and amino acid composition in haruan as a potential role in wound healing.

作者信息

Jais A M, McCulloch R, Croft K

机构信息

Department of Fisheries Biology, Faculty of Fisheries and Marine Sciences, University Pertanian Malaysia, Serdang, Selangor.

出版信息

Gen Pharmacol. 1994 Sep;25(5):947-50. doi: 10.1016/0306-3623(94)90101-5.

DOI:10.1016/0306-3623(94)90101-5
PMID:7835642
Abstract
  1. Two species of snakehead fish are available in Sabah, i.e. Channa striatus and Channa melanosoma, and are commonly known as haruan. Haruan is consumed by many Malaysians to induce healing after a clinical operations. However, there is no scientific evidence as yet to substantiate the claim, and so it was decided to analyse the biochemical composition in haruan to determine which compounds may have a possible role or potential in wound healing. 2. Samples (midline fillet) of both species were extracted separately in hexane for the qualitative analysis of fatty acids by a gas chromatography, Hewlett-Packard 5890A, using a 10 meter superox 11 column (Alltech) at temperature between 190 and 245 degrees C. Peak areas were calculated automatically using Hewlett-Packard 3393A computing integrator. Subsequently, the amino acid composition was analysed using a precolumn derivatization reverse phase HPLC waters PICO-TAG system. 3. Haruan is found to contain unusually high arachidonic acid (AA) but almost no eicosapentaenoic acid (EPA). AA which is a precursor of prostaglandin may initiate blood clotting and be responsible for growth. Haruan also contains all the essential amino acids for wound healing, particularly glycine which is the most important component of human skin collagen. Therefore, haruan contained all the basic biochemical requirements for wound healing.
摘要
  1. 沙巴有两种鳢鱼,即条纹鳢和黑体鳢,通常被称为哈鲁安。许多马来西亚人食用哈鲁安以促进临床手术后的愈合。然而,目前尚无科学证据证实这一说法,因此决定分析哈鲁安的生化成分,以确定哪些化合物可能在伤口愈合中发挥作用或具有潜在作用。2. 分别用己烷提取这两种鱼的样本(鱼身中线鱼片),通过惠普5890A气相色谱仪对脂肪酸进行定性分析,使用10米的超氧11柱(奥泰克),温度在190至245摄氏度之间。峰面积使用惠普3393A计算积分仪自动计算。随后,使用柱前衍生化反相高效液相色谱沃特世PICO-TAG系统分析氨基酸组成。3. 发现哈鲁安含有异常高的花生四烯酸(AA),但几乎不含二十碳五烯酸(EPA)。作为前列腺素前体的AA可能启动血液凝固并促进生长。哈鲁安还含有伤口愈合所需的所有必需氨基酸,特别是甘氨酸,它是人体皮肤胶原蛋白的最重要成分。因此,哈鲁安含有伤口愈合所需的所有基本生化成分。

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