从黄乳菇中纯化和鉴定一种具有抗增殖活性的新型核糖核酸酶。
Purification and characterization of a novel RNase with antiproliferative activity from the mushroom Lactarius flavidulus.
机构信息
State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing, China.
出版信息
J Antibiot (Tokyo). 2012 Feb;65(2):67-72. doi: 10.1038/ja.2011.112. Epub 2011 Dec 21.
A 14.6-kDa RNase, with a pH optimum of 5.5 and a temperature optimum of 70 °C, was isolated from dried fruiting bodies of the edible mushroom Lactarius flavidulus. The purification procedure involved, in succession, ion exchange chromatography on DEAE-cellulose, CM-cellulose and SP-Sepharose, and finally FPLC-gel filtration on Superdex 75. The RNase was adsorbed on all three ion exchangers. The ranking of its activity toward various polyhomoribonucleotides was poly(C) > poly(G) > poly(A) > poly(U). It suppressed proliferation of HepG2 cells and L1210 cells with an IC₅₀ of 3.19 μM and 6.52 μM, respectively. It also inhibited the activity of HIV-1 reverse transcriptase with an IC₅₀ of 2.55 μM.
一种 14.6 kDa 的 RNase，最适 pH 为 5.5，最适温度为 70°C，从可食用蘑菇乳菇的干燥子实体中分离得到。纯化过程依次包括 DEAE-纤维素、CM-纤维素和 SP-琼脂糖的离子交换层析，最后在 Superdex 75 上进行 FPLC-凝胶过滤。RNase 被三种离子交换剂吸附。它对各种多聚核糖核苷酸的活性排序为 poly(C) > poly(G) > poly(A) > poly(U)。它对 HepG2 细胞和 L1210 细胞的增殖具有抑制作用，IC₅₀值分别为 3.19 μM 和 6.52 μM。它还抑制 HIV-1 逆转录酶的活性，IC₅₀值为 2.55 μM。
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