Oh Jin Soo, An Sung Su, Gwak So-Jung, Pennant William A, Kim Keung Nyun, Yoon Do Heum, Ha Yoon
Department of Neurosurgery, Spine and Spinal Cord , College of Medicine, Yonsei University, Seoul, Korea.
Neuroreport. 2012 Feb 15;23(3):174-8. doi: 10.1097/WNR.0b013e32834f4f3a.
We established three stable neural stem cell (NSC) lines to explore the possibility of using hypoxia-specific vascular endothelial growth factor (VEGF) expressing NSC lines (EpoSV-VEGF NSCs) to treat spinal cord injury. The application of EpoSV-VEGF NSCs into the injured spinal cord after clip compression injury not only showed therapeutic effects such as extended survival and angiogenesis, but also displayed its safety profile as it did not cause unwanted cell proliferation or angiogenesis in normal spinal cord tissue, as EpoSV-VEGF NSCs consistently showed hypoxia-specific VEGF expression patterns. This suggests that our EpoSV-VEGF NSCs are both safe and therapeutically efficacious for the treatment of spinal cord injury. Furthermore, this hypoxia-inducible gene expression system may represent a safe tool suitable for gene therapy.
我们建立了三种稳定的神经干细胞（NSC）系，以探索使用表达缺氧特异性血管内皮生长因子（VEGF）的神经干细胞系（EpoSV-VEGF NSCs）治疗脊髓损伤的可能性。在夹闭压迫性损伤后将EpoSV-VEGF NSCs应用于损伤的脊髓，不仅显示出诸如延长存活和血管生成等治疗效果，还显示出其安全性，因为它不会在正常脊髓组织中引起不必要的细胞增殖或血管生成，因为EpoSV-VEGF NSCs始终显示出缺氧特异性VEGF表达模式。这表明我们的EpoSV-VEGF NSCs对于治疗脊髓损伤既安全又有效。此外，这种缺氧诱导基因表达系统可能代表一种适用于基因治疗的安全工具。
