序列测定和分析两种芜菁花叶病毒 NSs 基因。
Sequence determination and analysis of the NSs genes of two tospoviruses.
机构信息
Departamento de Biologia Celular, Universidade de Brasília, Brasilia, DF 70910-900, Brazil.
出版信息
Arch Virol. 2012 Mar;157(3):591-6. doi: 10.1007/s00705-011-1196-4. Epub 2011 Dec 21.
The tospoviruses groundnut ringspot virus (GRSV) and zucchini lethal chlorosis virus (ZLCV) cause severe losses in many crops, especially in solanaceous and cucurbit species. In this study, the non-structural NSs gene and the 5'UTRs of these two biologically distinct tospoviruses were cloned and sequenced. The NSs sequence of GRSV and ZLCV were both 1,404 nucleotides long. Pairwise comparison showed that the NSs amino acid sequence of GRSV shared 69.6% identity with that of ZLCV and 75.9% identity with that of TSWV, while the NSs sequence of ZLCV and TSWV shared 67.9% identity. Phylogenetic analysis based on NSs sequences confirmed that these viruses cluster in the American clade.
这些番茄斑萎病毒（GRSV）和南瓜曲叶病毒（ZLCV）给许多作物，特别是茄科和葫芦科作物造成严重损失。本研究克隆并测序了这两种具有明显生物学差异的番茄斑萎病毒的非结构 NSs 基因和 5'UTR。GRSV 和 ZLCV 的 NSs 序列均为 1404 个核苷酸长。序列比对显示，GRSV 的 NSs 氨基酸序列与 ZLCV 同源性为 69.6%，与 TSWV 同源性为 75.9%，而 ZLCV 和 TSWV 的 NSs 序列同源性为 67.9%。基于 NSs 序列的系统进化分析证实，这些病毒聚集在美洲分支。
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