Research Center for Gastroenterology and Liver Diseases-Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Gastrointestin Liver Dis. 2011 Dec;20(4):371-6.
A number of theories have been put forward to clarify the etiology of colorectal cancer (CRC), such as genetic alterations and cytokine production. A combination of inflammatory cytokines has an important role in cancer development. The aim of our study was to screen for alterations located in promoter and exons of IL-16 gene sequence, to determine the distribution of genotypes in individuals with CRC and healthy controls in a sample of Iranian population.
The case group consisted of 260 individuals with colorectal cancer and the control group included 405 healthy individuals. Three IL-16 gene polymorphisms (rs4072111, rs11556218, rs4778889) were genotyped using PCR-RFLP method. RFLP results were confirmed by direct sequencing.
A significant association between rs11556218 SNP in the IL-16 gene and the risk of CRC was found. The TG genotype of rs11556218 T/G polymorphism showed significant association with a 1.75 fold increased risk of CRC (P=0.005; adjusted OR: 1.759; 95% CI: 1.191-2.598). In addition a significant association between CC genotype of rs4778889 T/C polymorphism and decreased risk of CRC in male subjects (P=0.045; adjusted OR: 0.192; 95% CI: 0.038-0.967) was determined.
This study is the first report of IL-16 gene polymorphisms among CRC patients from Iran. Our results suggest an influence of rs11556218 T > G and rs4778889 T/C polymorphisms on the altered risk of CRC.
有许多理论被提出以阐明结直肠癌（CRC）的病因，如遗传改变和细胞因子的产生。炎性细胞因子的组合在癌症的发展中起着重要作用。我们的研究目的是筛选位于 IL-16 基因序列启动子和外显子中的改变，以确定伊朗人群中 CRC 患者和健康对照个体中基因型的分布。
病例组由 260 名结直肠癌患者组成，对照组包括 405 名健康个体。使用 PCR-RFLP 方法对 IL-16 基因的三个基因多态性（rs4072111、rs11556218、rs4778889）进行基因分型。通过直接测序确认 RFLP 结果。
IL-16 基因中的 rs11556218 SNP 与 CRC 的风险之间存在显著关联。rs11556218 T/G 多态性的 TG 基因型与 CRC 的风险增加 1.75 倍显著相关（P=0.005；调整后的 OR：1.759；95%CI：1.191-2.598）。此外，rs4778889 T/C 多态性的 CC 基因型与男性 CRC 风险降低之间存在显著关联（P=0.045；调整后的 OR：0.192；95%CI：0.038-0.967）。
本研究是伊朗 CRC 患者中首次报道的 IL-16 基因多态性。我们的研究结果表明，rs11556218 T > G 和 rs4778889 T/C 多态性对 CRC 风险的改变有影响。
