Ali Yasser B M, Saed Mai M, Abdel-Hakem Nehal E, Hussein Mona Abd Elmotaleb A, El-Shahat Mohamed
Molecular Immunology Division, Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat City, Egypt.
Molecular Biochemistry Division, Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute (GEBRI), University of Sadat City, Sadat City, Egypt.
Immunol Invest. 2024 Jul;53(5):830-842. doi: 10.1080/08820139.2024.2349034. Epub 2024 May 21.
Type 1 diabetes (T1D) is a serious chronic autoimmune condition. Even though the underlying reason for the onset of T1D is unknown, due to their effector and regulatory roles in immune responses, cytokines are essential in developing autoimmune disorders. Interleukin (IL)16 is an immunomodulatory cytokine implicated in several inflammatory and autoimmune diseases.
This study was designed to examine the association of IL16 gene polymorphisms, rs11556218 T > G and rs4778889 T > C, with the risk of T1D in Egyptian children.
Using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay, we analyzed rs11556218 T > G and rs4778889 T > C polymorphisms of the IL16 gene in 100 T1D subjects and 93 controls.
Rs11556218 T > G polymorphism of the IL16 gene was not associated with the risk of developing T1D. Analysis of IL16 gene rs4778889 T > C showed that the TT genotype had a considerably higher risk of T1D than the TC genotype [OR = 2.195 (1.205-3.999)]. In comparison to patients with the C allele [OR = 0.6914 (0.38-1.2569)], patients with the T allele [OR = 1.45 (0.7956-2.6296)] were notably more likely to have T1D. A significant decrease was found in the frequency of GT (OR = 0.43, = .03) and TC (OR = 0.32, = .011) haplotypes of IL16 gene rs11556218 T > G and rs4778889 T > C polymorphisms in T1D patients compared with controls.
IL16 gene rs4778889 T > C polymorphism might be associated with susceptibility to T1D. Egyptians with TT genotypes are more likely to develop T1D. However, GT and TC haplotypes of IL16 gene rs11556218 T > G and rs4778889 T > C polymorphisms highlight their protective role againstT1D disease.
1型糖尿病（T1D）是一种严重的慢性自身免疫性疾病。尽管T1D发病的根本原因尚不清楚，但由于细胞因子在免疫反应中具有效应和调节作用，它们在自身免疫性疾病的发展中至关重要。白细胞介素（IL）-16是一种免疫调节细胞因子，与多种炎症和自身免疫性疾病有关。
本研究旨在探讨IL-16基因多态性rs11556218 T>G和rs4778889 T>C与埃及儿童患T1D风险的相关性。
采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析方法，对100例T1D患者和93例对照者的IL-16基因rs11556218 T>G和rs4778889 T>C多态性进行分析。
IL-16基因的rs11556218 T>G多态性与患T1D的风险无关。对IL-16基因rs4778889 T>C的分析表明，TT基因型患T1D的风险显著高于TC基因型[比值比（OR）=2.195（1.205-3.999）]。与携带C等位基因的患者相比[OR=0.6914（0.38-1.2569）]，携带T等位基因的患者患T1D的可能性明显更高[OR=1.45（0.7956-2.6296）]。与对照组相比，T1D患者中IL-16基因rs11556218 T>G和rs4778889 T>C多态性的GT单倍型（OR=0.43，P=0.03）和TC单倍型（OR=0.32，P=0.011）频率显著降低。
IL-16基因rs4778889 T>C多态性可能与T1D易感性有关。TT基因型的埃及人患T1D的可能性更大。然而，IL-16基因rs11556218 T>G和rs4778889 T>C多态性的GT和TC单倍型显示出它们对T1D疾病的保护作用。
