Department of Dermatology, University Hospital, Technical University Dresden, Dresden, Germany.
Clin Exp Dermatol. 2012 Mar;37(2):169-76. doi: 10.1111/j.1365-2230.2011.04213.x. Epub 2011 Dec 20.
The immune system of the skin has a network of resident dendritic cells (DCs) consisting of epidermal Langerhans cells and various subsets of dermal DCs. We recently reported on a new population of dermal DCs, called slan (6-sulfoLacNAc+) DCs, which have a potent capacity to stimulate Th17/Th1 T-cell responses.
To understand the characteristics of slanDCs as a new population of dermal DCs in the context of other DC populations in healthy and psoriatic skin.
We immunofluorescently stained skin samples from healthy controls and from patients with psoriasis.
Staining healthy skin for DCs showed that slanDCs (CD1a- CD1c- CD11c- CD14- CD163-) were present at a similar frequency to that of CD1c+ CD11c+ CD1a+ CD14- CD163- dermal DCs, which have previously been regarded as the major population of resident DCs. In psoriatic skin, the frequency of slanDCs and CD1c+ DCs was doubled, and the slanDCs expressed CD11c. In-depth analysis of DCs in psoriatic skin by four-colour immunofluorescence analysis showed that the pool of CD11c+ cells could be further subdivided into CD11c+ CD14+ CD163- DCs and CD11c+ CD163+ CD14+ macrophages.
SlanDCs, initially described as large population of proinflammatory DCs in blood, are a novel and major part of the resident dermal myeloid DC system in both healthy and inflamed skin.
皮肤的免疫系统具有由表皮朗格汉斯细胞和各种真皮树突状细胞亚群组成的常驻树突状细胞（DC）网络。我们最近报道了一种新的真皮 DC 群体，称为 slan（6-磺基 LacNAc+）DC，其具有刺激 Th17/Th1 T 细胞反应的强大能力。
了解 slanDC 作为健康和银屑病皮肤中其他 DC 群体背景下的一种新型真皮 DC 群体的特征。
我们免疫荧光染色了来自健康对照者和银屑病患者的皮肤样本。
对健康皮肤的 DC 进行染色显示，slanDC（CD1a-CD1c-CD11c-CD14-CD163-）与以前被认为是主要常驻 DC 群体的 CD1c+CD11c+CD1a+CD14-CD163-真皮 DC 具有相似的频率。在银屑病皮肤中，slanDC 和 CD1c+DC 的频率增加了一倍，并且 slanDC 表达 CD11c。通过四色免疫荧光分析对银屑病皮肤中的 DC 进行深入分析显示，CD11c+细胞池可进一步细分为 CD11c+CD14+CD163-DC 和 CD11c+CD163+CD14+巨噬细胞。
最初在血液中描述为大型促炎 DC 群体的 slanDC 是健康和炎症皮肤中常驻真皮髓样 DC 系统的新型主要组成部分。
