Passarelli M K, Winograd N
Department of Chemistry, The Pennsylvania State University, 104 Chemistry Building, University Park, Pennsylvania 16802, USA.
Surf Interface Anal. 2011 Jan;43(1-2):269-271. doi: 10.1002/sia.3529.
The dual functionality of a C(60) (+)-Q-Star hybrid instrument allows for the examination of complex lipid profiles with both MALDI and SIMS methodologies.([1]) The difficulties associated with characterizing lipids in situ - isobaric interference and salt contamination - are discussed. The inherent advantages of both methodologies were used to deconvolute complex lipid spectra and establish characteristic fragmentation pathways. The high lateral resolution of SIMS allows for single cell images of aplysia californica neurons, while the established protocols were utilized to identify a major lipid component.
C(60) (+)-Q-Star 混合仪器的双重功能使得能够使用基质辅助激光解吸/电离（MALDI）和二次离子质谱（SIMS）方法来检测复杂的脂质谱。（[1]）讨论了原位表征脂质所涉及的困难——等压干扰和盐污染。利用这两种方法的固有优势对复杂的脂质谱进行去卷积，并建立特征性的裂解途径。SIMS 的高横向分辨率能够获得加州海兔神经元的单细胞图像，同时利用既定方案鉴定出一种主要的脂质成分。
