Passarelli Melissa K, Winograd Nicholas
Department of Chemistry, Pennsylvania State University, University Park, PA, USA.
Biochim Biophys Acta. 2011 Nov;1811(11):976-90. doi: 10.1016/j.bbalip.2011.05.007. Epub 2011 May 27.
Fundamental advances in secondary ion mass spectrometry (SIMS) now allow for the examination and characterization of lipids directly from biological materials. The successful application of SIMS-based imaging in the investigation of lipids directly from tissue and cells are demonstrated. Common complications and technical pitfalls are discussed. In this review, we examine the use of cluster ion sources and cryogenically compatible sample handling for improved ion yields and to expand the application potential of SIMS. Methodological improvements, including pre-treating the sample to improve ion yields and protocol development for 3-dimensional analyses (i.e. molecular depth profiling), are also included in this discussion. New high performance SIMS instruments showcasing the most advanced instrumental developments, including tandem MS capabilities and continuous ion beam compatibility, are described and the future direction for SIMS in lipid imaging is evaluated.
二次离子质谱（SIMS）的基础性进展如今使得直接从生物材料中检测和表征脂质成为可能。基于SIMS的成像技术在直接从组织和细胞中研究脂质方面的成功应用得到了证实。文中还讨论了常见的并发症和技术陷阱。在本综述中，我们探讨了使用簇离子源和低温兼容的样品处理方法来提高离子产率，并扩大SIMS的应用潜力。讨论还包括方法学的改进，如对样品进行预处理以提高离子产率，以及三维分析（即分子深度剖析）的方案开发。文中描述了展示最先进仪器发展的新型高性能SIMS仪器，包括串联质谱功能和连续离子束兼容性，并评估了SIMS在脂质成像方面的未来发展方向。
