Pundir Chandra Shekhar, Rawal Rachna, Chawla Sheetal, Kuhad Ramesh Chandra
Department of Biochemistry, MD University, Rohtak, India.
Artif Cells Blood Substit Immobil Biotechnol. 2012 Feb;40(1-2):163-70. doi: 10.3109/10731199.2011.637926. Epub 2011 Dec 22.
A method is described for construction of an amperometric polyphenol biosensor employing nitrocellulose membrane-bound laccase purified from cell-free extract of Ganoderma lucidum onto a Pt electrode. The biosensor showed optimum response within 10s, at 0.4 V in 0.1M acetate buffer, pH 6.0, and 35°C. Detection limit of the biosensor was 3.0 × 10(-8)M. Analytical recovery of added guaiacol was 97.00%. Within batch and between batch coefficients of variation were <0.97% and <1.26%, respectively. The sensor measured total phenolic content in fruit juices and alcoholic beverages. The enzyme electrode was used 100 times over 4 months, when stored at 4°C.
本文描述了一种构建电流型多酚生物传感器的方法，该方法是将从灵芝无细胞提取物中纯化得到的漆酶固定在硝酸纤维素膜上，再将其置于铂电极上。该生物传感器在0.1M醋酸盐缓冲液（pH 6.0）、35°C条件下，于0.4 V电位时，10秒内显示出最佳响应。该生物传感器的检测限为3.0×10(-8)M。添加愈创木酚的分析回收率为97.00%。批内和批间变异系数分别<0.97%和<1.26%。该传感器可测定果汁和酒精饮料中的总酚含量。当在4°C储存时，该酶电极在4个月内可使用100次。
