Department of Biochemistry, M.D. University, Rohtak 124 001, Haryana, India.
J Biotechnol. 2011 Oct 20;156(1):39-45. doi: 10.1016/j.jbiotec.2011.08.008. Epub 2011 Aug 16.
A high-performance amperometric polyphenol biosensor was developed, based on covalent immobilization of Ganoderma sp. laccase onto copper nanoparticles (CuNP's)/chitosan (CHIT)/carboxylated multiwalled carbon nanotube (cMWCNT)/polyaniline (PANI)-modified gold (Au) electrode. The CuNP's and cMWCNT had a synergistic electrocatalytic effect in the matrix of CHIT. The biosensor showed optimum response at pH 6.0 (0.1 M acetate buffer) and 35°C, when operated at 50 mVs(-1). The biosensor exhibited excellent sensitivity (the detection limit was down to 0.156 μM for guaiacol), fast response time (less than 4s) and wide linear range (from 1 to 500 μM). Analytical recovery of added guaiacol was 96.40-98.46%. Within batch and between batch coefficients of variation were <2.6% and <5.3%, respectively. The enzyme electrode was used 300 times over a period of 7 months, when stored at 4°C.
基于灵芝漆酶共价固定在铜纳米粒子（CuNP）/壳聚糖（CHIT）/羧基多壁碳纳米管（cMWCNT）/聚苯胺（PANI）修饰金（Au）电极上，研制了一种高性能的多酚安培生物传感器。CuNP 和 cMWCNT 在 CHIT 基质中具有协同的电催化作用。该生物传感器在 pH 6.0（0.1 M 醋酸盐缓冲液）和 35°C 下，在 50 mVs(-1)时表现出最佳响应。该生物传感器具有出色的灵敏度（检测限低至 0.156 μM 对愈创木酚）、快速的响应时间（<4s）和较宽的线性范围（1-500 μM）。添加愈创木酚的分析回收率为 96.40-98.46%。批内和批间变异系数分别小于 2.6%和 5.3%。酶电极在 4°C 下储存 7 个月期间，可使用 300 次。
