Department of Cardiology, Institute of Cardiovascular Diseases, First Affiliated Hospital, Guangxi Medical University, 22 Shuangyong Road, Nanning 530021, Guangxi, People's Republic of China.
Lipids Health Dis. 2011 Dec 23;10:242. doi: 10.1186/1476-511X-10-242.
The single nucleotide polymorphism (SNP) of peroxisome proliferator-activated receptor delta (PPARD) gene affects serum lipid profiles, but to what extent alcohol consumption interferes with this association remains unknown. The present study was undertaken to compare the association of PPARD +294T > C (rs2016520) polymorphism and serum lipid levels in the nondrinkers and drinkers.
A total of 685 unrelated nondrinkers and 497 drinkers aged 15-82 were randomly selected from our previous stratified randomized cluster samples. Genotyping of the PPARD +294T > C was performed by polymerase chain reaction and restriction fragment length polymorphism. Interactions of the PPARD +294T > C genotypes and alcohol consumption on serum lipid levels were detected by using a factorial regression analysis after controlling for potential confounders.
The levels of triglyceride (TG), high-density lipoprotein cholesterol (HDL-C), apolipoprotein (Apo) A1, and the ratio of ApoA1 to ApoB were higher in drinkers than in nondrinkers (P < 0.05-0.001). There were no significant differences in the levels of total cholesterol (TC), low-density lipoprotein cholesterol (LDL-C) and ApoB between the two groups (P > 0.05 for all). The frequencies of TT, TC and CC genotypes were 56.0%, 36.4% and 7.6% in nondrinkers, and 57.2%, 38.0% and 4.8% in drinkers (P > 0.05); respectively. The frequencies of T and C alleles were 74.2% and 25.8% in nondrinkers, and 76.2% and 23.8% in drinkers (P > 0.05); respectively. There was also no significant difference in the genotypic and allelic frequencies between males and females in both groups (P > 0.05 for all). The levels of TC in nondrinkers were different among the three genotypes (P = 0.01), the C allele carriers had higher serum TC levels than the C allele noncarriers. The levels of all seven lipid traits in drinkers were not different among the three genotypes (P > 0.05 for all). The interactions of PPARD +294T > C genotypes and alcohol consumption on serum lipid levels were not detected in the drinkers (P >0.05 for all). Multiple linear regression analysis showed that serum TC, HDL-C, LDL-C, ApoA1, and ApoB levels were correlated with genotypes in drinkers but not in nondrinkers (P < 0.05-0.01).
These results suggest that the great majority of our study populations are beneficial from alcohol consumption. But there is no interaction between the PPARD +294T > C genotypes and alcohol consumption on serum lipid levels in the drinkers.
过氧化物酶体增殖物激活受体 δ（PPARD）基因的单核苷酸多态性（SNP）影响血清脂质谱，但饮酒在多大程度上干扰这种关联尚不清楚。本研究旨在比较 PPARD+294T>C（rs2016520）多态性与非饮酒者和饮酒者血清脂质水平的关联。
从我们之前的分层随机聚类样本中随机选择了 685 名非饮酒者和 497 名饮酒者（年龄 15-82 岁）。通过聚合酶链反应和限制性片段长度多态性对 PPARD+294T>C 进行基因分型。在控制潜在混杂因素后，使用因子回归分析检测 PPARD+294T>C 基因型和饮酒之间对血清脂质水平的交互作用。
饮酒者的甘油三酯（TG）、高密度脂蛋白胆固醇（HDL-C）、载脂蛋白（Apo）A1 和 ApoA1 与 ApoB 的比值高于非饮酒者（P<0.05-0.001）。两组间总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-C）和 ApoB 水平无显著差异（均 P>0.05）。非饮酒者 TT、TC 和 CC 基因型的频率分别为 56.0%、36.4%和 7.6%，饮酒者分别为 57.2%、38.0%和 4.8%（均 P>0.05）；非饮酒者 T 和 C 等位基因的频率分别为 74.2%和 25.8%，饮酒者分别为 76.2%和 23.8%（均 P>0.05）。两组中男性和女性之间基因型和等位基因频率也无显著差异（均 P>0.05）。非饮酒者中 TC 水平在三种基因型之间存在差异（P=0.01），C 等位基因携带者的血清 TC 水平高于非 C 等位基因携带者。饮酒者中七种脂质特征的水平在三种基因型之间无差异（均 P>0.05）。在饮酒者中，未检测到 PPARD+294T>C 基因型和饮酒对血清脂质水平的交互作用（均 P>0.05）。多元线性回归分析显示，饮酒者的血清 TC、HDL-C、LDL-C、ApoA1 和 ApoB 水平与基因型相关，但在非饮酒者中则无相关（均 P<0.05-0.01）。
这些结果表明，我们研究人群中的绝大多数都从饮酒中获益。但在饮酒者中，PPARD+294T>C 基因型与饮酒之间对血清脂质水平无交互作用。
