The Dow Chemical Company, 2301 N. Brazosport Blvd, Freeport, TX 77541-3257, USA.
J Chromatogr A. 2012 Feb 24;1226:116-23. doi: 10.1016/j.chroma.2011.11.036. Epub 2011 Dec 4.
A simple and reliable differential flow modulator has been demonstrated which reverses the flow during the flush step. The modulator is constructed with commercially available capillary flow technology tees which simplifies the apparatus and permits wide range of column dimensions to be used because the modulator volume is adjustable. Using a reverse flush arrangement the tailing of the peak at the base (baseline rise between modulations) is reduced 10-20 fold as compared to forward flush modulation. This is most easily observed for peaks overloaded in the first dimension. Excellent reproducibility (<2% RSD) of area measurements has been demonstrated with a complex fragrance sample as well as the capacity to handle significant overloading without loss of resolution in the second dimension. Further demonstrating the flexibility of this modulator, separation of C1-6 alkanes and olefins are demonstrated with two porous layer open tubular columns.
已经证明，一种简单可靠的差分流量调制器可以在冲洗步骤中反转流。该调制器由市售的毛细管流动技术三通构建而成，这简化了仪器，并允许使用广泛的柱尺寸，因为调制器体积是可调节的。使用反向冲洗布置，与正向冲洗调制相比，在基线上（调制之间的基线上升）的峰的尾部减少了 10-20 倍。对于在第一维上过载的峰，这是最容易观察到的。已经用复杂的香料样品证明了面积测量的出色重现性（<2%RSD），并且能够在不损失第二维分辨率的情况下处理显著的过载。进一步证明了这种调制器的灵活性，用两个多孔层开管柱演示了 C1-6 烷烃和烯烃的分离。
