Joint Program in Bioengineering, University of Texas-Southwestern Medical Center at Dallas and The University of Texas at Arlington, Arlington, Texas, United States of America.
PLoS One. 2011;6(12):e28720. doi: 10.1371/journal.pone.0028720. Epub 2011 Dec 14.
In recent years, research efforts exploring the possibility of using biomaterial nanoparticles for intravitreous drug delivery has increased significantly. However, little is known about the effect of material properties on intravitreous tissue responses.
To find the answer, nanoparticles made of hyaluronic acid (HA), poly (l-lactic acid) (PLLA), polystyrene (PS), and Poly N-isopropyl acrylamide (PNIPAM) were tested using intravitreous rabbit implantation model. Shortly after implantation, we found that most of the implants accumulated in the trabecular meshwork area followed by clearance from the vitreous. Interestingly, substantial reduction of intraocular pressure (IOP) was observed in eyes implanted with particles made of PS, PNIPAM and PLLA, but not HA nanoparticles and buffered salt solution control. On the other hand, based on histology, we found that the particle implantation had no influence on cornea, iris and even retina. Surprisingly, substantial CD11b+ inflammatory cells were found to accumulate in the trabecular meshwork area in some animals. In addition, there was a good relationship between recruited CD11b+ cells and IOP reduction.
Overall, the results reveal the potential influence of nanoparticle material properties on IOP reduction and inflammatory responses in trabecular meshwork. Such interactions may be critical for the development of future ocular nanodevices with improved safety and perhaps efficacy.
近年来，研究人员探索使用生物材料纳米颗粒进行玻璃体内药物输送的可能性的工作显著增加。然而，对于材料特性对玻璃体内组织反应的影响知之甚少。
为了找到答案，使用玻璃体内兔植入模型测试了由透明质酸（HA）、聚（L-丙交酯）（PLLA）、聚苯乙烯（PS）和聚 N-异丙基丙烯酰胺（PNIPAM）制成的纳米颗粒。植入后不久，我们发现大多数植入物积聚在小梁网区域，随后从玻璃体内清除。有趣的是，植入 PS、PNIPAM 和 PLLA 制成的颗粒的眼睛的眼内压（IOP）显著降低，但 HA 纳米颗粒和缓冲盐溶液对照则没有。另一方面，根据组织学，我们发现颗粒植入对角膜、虹膜甚至视网膜没有影响。令人惊讶的是，在一些动物中发现大量 CD11b+炎症细胞积聚在小梁网区域。此外，招募的 CD11b+细胞与 IOP 降低之间存在良好的关系。
总体而言，结果揭示了纳米颗粒材料特性对小梁网中 IOP 降低和炎症反应的潜在影响。这种相互作用对于开发具有改善安全性和疗效的未来眼纳米器件可能至关重要。
