State key Laboratory of Ophthalmology, Zhongshan Ophthalmic Center, Sun Yat-sen University, Guangzhou, Guangdong, China.
Clin Exp Ophthalmol. 2012 Jan-Feb;40(1):e67-75. doi: 10.1111/j.1442-9071.2011.02664.x. Epub 2011 Oct 20.
The aim of this study was to evaluate over a 180-day period the biocompatibility and retinal support of a foldable capsular vitreous body injected with either saline or silicone oil implanted in rabbit eyes.
A standard three-port pars plana vitrectomy was performed, and foldable capsular vitreous bodies were implanted into the vitreous cavity of rabbit eyes (n = 18). Silicone oil tamponade was used as the control group (n = 5). Of the foldable capsular vitreous body-implanted eyes, either saline (n = 9) or silicone oil (n = 9) was injected into the foldable capsular vitreous body to support the retina. The treated eyes were examined using a slit lamp with a non-contact slit-lamp lens, a tonopen, a non-contact specular microscope and a B-scan ultrasound during the 180-day implantation period. A histological examination was performed at 90 and 180 days.
During the 180-day implantation period, no significant corneal keratopathy or intraocular inflammation was noted, and the intraocular pressure (IOP) and corneal endothelial numbers remained steady among the three groups. B-scan ultrasonography showed a smoothly increased echogenicity in front of the retina in group of foldable capsular vitreous bodies injected with saline. Gross examination showed that the foldable capsular vitreous bodies injected with saline or silicone oil smoothly supported the retina. The saline or silicone oil inside the foldable capsular vitreous body was homogeneous, transparent and filled the foldable capsular vitreous body. Histological examination showed no obvious abnormality of the cornea, ciliary body or retina in the foldable capsular vitreous body-implanted eyes.
These results suggest that foldable capsular vitreous bodies injected with either saline or silicone oil showed good biocompatibility and retinal support in rabbit eyes over a 180-day implantation time.
本研究旨在评估在 180 天的时间内，向兔眼内注入生理盐水或硅油的可折叠囊玻璃体的生物相容性和视网膜支持作用。
进行标准的三通道经睫状体平坦部玻璃体切除术，将可折叠囊玻璃体植入兔眼的玻璃体腔（n=18）。硅油填充作为对照组（n=5）。在植入可折叠囊玻璃体的眼中，向可折叠囊玻璃体中注入生理盐水（n=9）或硅油（n=9）以支持视网膜。在 180 天的植入期间，使用带有非接触式裂隙灯镜头、眼压计、非接触式共焦显微镜和 B 型超声扫描仪检查治疗眼。在第 90 和 180 天进行组织学检查。
在 180 天的植入期间，三组均未出现明显的角膜混浊或眼内炎症，眼内压（IOP）和角膜内皮细胞数量保持稳定。B 型超声检查显示，向可折叠囊玻璃体中注入生理盐水的眼，视网膜前的回声逐渐增强。大体检查显示，向可折叠囊玻璃体中注入生理盐水或硅油的眼，可使视网膜得到平滑的支撑。可折叠囊玻璃体中的生理盐水或硅油均匀、透明，充满可折叠囊玻璃体。组织学检查显示，植入可折叠囊玻璃体的眼中，角膜、睫状体或视网膜无明显异常。
这些结果表明，在兔眼中，向可折叠囊玻璃体中注入生理盐水或硅油在 180 天的植入时间内均表现出良好的生物相容性和视网膜支持作用。
