pharmazentrum frankfurt/ZAFES, Institut für Klinische Pharmakologie, Klinikum der Goethe-Universität, Frankfurt am Main, Germany.
PLoS One. 2011;6(12):e29240. doi: 10.1371/journal.pone.0029240. Epub 2011 Dec 14.
Accumulating evidence indicates that increased generation of reactive oxygen species (ROS) contributes to the development of exaggerated pain hypersensitivity during persistent pain. In the present study, we investigated the antinociceptive efficacy of the antioxidants vitamin C and vitamin E in mouse models of inflammatory and neuropathic pain. We show that systemic administration of a combination of vitamins C and E inhibited the early behavioral responses to formalin injection and the neuropathic pain behavior after peripheral nerve injury, but not the inflammatory pain behavior induced by Complete Freund's Adjuvant. In contrast, vitamin C or vitamin E given alone failed to affect the nociceptive behavior in all tested models. The attenuated neuropathic pain behavior induced by the vitamin C and E combination was paralleled by a reduced p38 phosphorylation in the spinal cord and in dorsal root ganglia, and was also observed after intrathecal injection of the vitamins. Moreover, the vitamin C and E combination ameliorated the allodynia induced by an intrathecally delivered ROS donor. Our results suggest that administration of vitamins C and E in combination may exert synergistic antinociceptive effects, and further indicate that ROS essentially contribute to nociceptive processing in special pain states.
越来越多的证据表明，活性氧（ROS）的产生增加导致持续性疼痛期间疼痛过敏的加剧。在本研究中，我们研究了抗氧化剂维生素 C 和维生素 E 在炎症和神经病理性疼痛小鼠模型中的镇痛功效。我们发现，维生素 C 和 E 的联合系统给药抑制了福尔马林注射后的早期行为反应和外周神经损伤后的神经病理性疼痛行为，但不抑制完全弗氏佐剂诱导的炎症性疼痛行为。相比之下，单独给予维生素 C 或维生素 E 均不能影响所有测试模型中的痛觉行为。维生素 C 和 E 联合减轻的神经病理性疼痛行为伴随着脊髓和背根神经节中 p38 磷酸化的减少，并且在鞘内给予这些维生素后也观察到这种现象。此外，维生素 C 和 E 的联合改善了鞘内给予 ROS 供体引起的感觉异常。我们的结果表明，维生素 C 和 E 的联合给药可能发挥协同镇痛作用，并进一步表明 ROS 本质上有助于特殊疼痛状态下的伤害感受处理。
