Şanlıurfa Viranşehir State Hospital, Şanlıurfa, Turkey.
Department of Orthopaedics and Traumatology, Çukurova University Faculty of Medicine, Adana, Turkey.
Acta Orthop Traumatol Turc. 2023 Jan;57(1):23-29. doi: 10.5152/j.aott.2023.22121.
This study aimed to investigate the clinical, electrophysiological, and histomorphological effects of local use of coenzyme Q10 and vitamin E combination in a rat model of peripheral nerve injury.
Forty adult female Wistar-Albino rats weighing 250-350 g were kept in a room with a temperature of 20-22°C and a light/dark cycle of 12 hours. They had free access to food and water. The right sciatic nerves of 40 rats were transected and repaired. Subjects were divided into 4 groups: controls (control-4 weeks and control-8 weeks) and treatments (treatment-4 weeks and treatment-8 weeks). A combination of coenzyme Q10 and vitamin E was applied to the repair site by a catheter placed subcutaneously in the treatment group. Only transection-repair was done in the control group. All groups were divided into 2 subgroups for histomorphological, clinical, and electrophysiological experiments because of concerns about possible interference with histomorphological preparation (5 rats in each group). The experiment results were examined by the thermal plantar test, action potential and latency time measurements, and electron microscopy at the end of 4 and 8 weeks. The intact group was studied as the uninterrupted 10 left sciatic nerves of control for 4 weeks.
The mean thermal plantar test results of the intact group were better than those of the control groups (P < .05). However, there was no significant difference between the intact and treatment groups. In the histomorphological examination, the number of myelinated axons increased significantly, and the myelin structure was closer to that of the intact group, especially when the treatment-8 group was compared with the control groups (control-4: P < .0001, control-8: P < .01).
Local use of coenzyme Q10 and vitamin E seems useful in the experimental rat sciatic nerve transection-repair model.
本研究旨在探讨辅酶 Q10 和维生素 E 联合局部应用于周围神经损伤大鼠模型的临床、电生理和组织形态学效果。
40 只成年雌性 Wistar-Albino 大鼠，体重 250-350 克，饲养在温度为 20-22°C、光照/黑暗周期为 12 小时的房间内。它们可以自由获得食物和水。40 只大鼠的右侧坐骨神经被横断并修复。将研究对象分为 4 组：对照组（对照组 4 周和对照组 8 周）和治疗组（治疗组 4 周和治疗组 8 周）。治疗组通过皮下放置的导管将辅酶 Q10 和维生素 E 的混合物应用于修复部位。对照组仅进行横断修复。由于担心可能会干扰组织形态学准备，所有组均被分为 2 个亚组进行组织形态学、临床和电生理学实验（每组 5 只大鼠）。在第 4 和第 8 周结束时，通过足底热觉测试、动作电位和潜伏期时间测量以及电子显微镜检查来检查实验结果。完整组是作为对照组 4 周的未中断的 10 只左侧坐骨神经进行研究的。
完整组的平均足底热觉测试结果优于对照组（P <.05）。然而，完整组和治疗组之间没有显著差异。在组织形态学检查中，髓鞘轴突数量明显增加，髓鞘结构更接近完整组，尤其是治疗 8 组与对照组相比时（对照组 4：P <.0001，对照组 8：P <.01）。
辅酶 Q10 和维生素 E 的局部应用在实验性大鼠坐骨神经横断修复模型中似乎是有用的。
