Department of Orthodontics, Peking University School and Hospital of Stomatology, Beijing, China.
Am J Orthod Dentofacial Orthop. 2012 Jan;141(1):30-40. doi: 10.1016/j.ajodo.2011.06.035.
In orthodontic treatment, teeth can relapse after tooth movement without retention. The aim of this study was to evaluate the inhibition effects of local osteoprotegerin (OPG) gene transfer on orthodontic relapse.
Eighteen male Wistar rats were divided into 3 groups. The maxillary right first molars of all animals were subjected to orthodontic force and moved mesially. Three weeks later, the force was removed, and the teeth relapsed. During the 2-week relapse period, the 3 groups of rats received local OPG gene transfer (experimental group), mock vector transfer (mock group), and no injections (control group). Tooth movement and relapse were measured by using palatal superimpositions of 3-dimensional digital models. Histomorphometric analysis was used to quantify osteoclasts, and microcomputed tomography analysis was done to quantify the alveolar bone and the tibia.
Relapse was significantly inhibited and the number of osteoclasts was reduced in the experimental group. On the other hand, bone mineral density and bone volume fraction of alveolar bone were significantly increased. Bone mineral density and bone volume fraction of the tibia showed no significant difference between the groups.
Local OPG gene transfer to periodontal tissues could inhibit relapse after orthodontic tooth movement, through the inhibition of osteoclastogenesis.
在正畸治疗中，牙齿在没有保持的情况下移动后可能会复发。本研究旨在评估局部骨保护素（OPG）基因转移对正畸复发的抑制作用。
将 18 只雄性 Wistar 大鼠分为 3 组。所有动物的上颌右侧第一磨牙均受到正畸力的作用而向近中移动。3 周后，去除力，牙齿复发。在 2 周的复发期间，3 组大鼠分别接受局部 OPG 基因转移（实验组）、模拟载体转移（模拟组）和无注射（对照组）。通过腭部三维数字模型叠加测量牙齿移动和复发情况。组织形态计量学分析用于定量破骨细胞，微计算机断层扫描分析用于定量牙槽骨和胫骨。
实验组的复发明显受到抑制，破骨细胞数量减少。另一方面，牙槽骨的骨矿物质密度和骨体积分数显著增加。各组之间胫骨的骨矿物质密度和骨体积分数无显著差异。
局部牙周组织 OPG 基因转移可通过抑制破骨细胞生成来抑制正畸牙齿移动后的复发。
