Netherlands Institute for Neuroscience, 1105 BA Amsterdam, The Netherlands.
Neuron. 2011 Dec 22;72(6):1012-24. doi: 10.1016/j.neuron.2011.10.015.
During brain development, before sensory systems become functional, neuronal networks spontaneously generate repetitive bursts of neuronal activity, which are typically synchronized across many neurons. Such activity patterns have been described on the level of networks and cells, but the fine-structure of inputs received by an individual neuron during spontaneous network activity has not been studied. Here, we used calcium imaging to record activity at many synapses of hippocampal pyramidal neurons simultaneously to establish the activity patterns in the majority of synapses of an entire cell. Analysis of the spatiotemporal patterns of synaptic activity revealed a fine-scale connectivity rule: neighboring synapses (<16 μm intersynapse distance) are more likely to be coactive than synapses that are farther away from each other. Blocking spiking activity or NMDA receptor activation revealed that the clustering of synaptic inputs required neuronal activity, demonstrating a role of developmentally expressed spontaneous activity for connecting neurons with subcellular precision.
在大脑发育过程中，在感觉系统发挥功能之前，神经元网络会自发产生重复的神经元活动爆发，这些活动通常在许多神经元中是同步的。这种活动模式已经在网络和细胞水平上进行了描述，但在自发网络活动期间单个神经元接收的输入的精细结构尚未得到研究。在这里，我们使用钙成像技术同时记录海马锥体神经元的许多突触的活动，以建立整个细胞中大多数突触的活动模式。对突触活动的时空模式进行分析，揭示了一种精细的连接规则：相邻的突触（突触间距离<16μm）比彼此相距较远的突触更有可能同时活动。阻断尖峰活动或 NMDA 受体激活表明，突触输入的聚类需要神经元活动，这表明发育表达的自发性活动在以亚细胞精度连接神经元方面发挥了作用。
