Zakaria Z A, Rofiee M S, Mohamed A M, Teh L K, Salleh M Z
Department of Biomedical Science, Universiti Putra Malaysia, Selangor, Malaysia.
J Acupunct Meridian Stud. 2011 Dec;4(4):248-56. doi: 10.1016/j.jams.2011.09.016. Epub 2011 Oct 19.
The present study aims to determine the in vitro antiproliferative and antioxidant activities of various extracts from the leaves of Melastoma malabathricum using various established in vitro assays. The aqueous extract inhibited the proliferation of Caov-3 and HL-60 cell lines, while the chloroform extract exhibited antiproliferative activity against the Caov-3, HL-60, and CEM-SS cell lines. The methanol extract demonstrated antiproliferative activity against more cell lines, including the MCF-7, HeLa, Caov-3, HL-60, CEM-SS, and MDA-MB-231 cancer cell lines. Interestingly, all extracts did not inhibit the proliferation of 3T3 cells, thus indicating their noncytotoxic properties. Unlike the chloroform extracts, the aqueous and methanol extracts of M malabathricum (20, 100, and 500 μg/ml) produced high antioxidant activity for the superoxide scavenging assay with only the 500 μg/ml aqueous and methanol extracts exhibited high activity for the 2,2-diphenyl -1-picrylhydrazyl radical scavenging assay. The total phenolic content recorded for the aqueous, methanol, and chloroform extracts were 3344.2 ± 19.1, 3055.1 ± 8.7, and 92.5 ± 7.3 mg/100 g of gallic acid, respectively. The M malabathricum leaves possessed potential antiproliferative and antioxidant activities that could be attributed to its high content of phenolic compounds.
本研究旨在通过各种已建立的体外试验，确定多花野牡丹叶片不同提取物的体外抗增殖和抗氧化活性。水提取物抑制了Caov-3和HL-60细胞系的增殖，而氯仿提取物对Caov-3、HL-60和CEM-SS细胞系表现出抗增殖活性。甲醇提取物对更多细胞系表现出抗增殖活性，包括MCF-7、HeLa、Caov-3、HL-60、CEM-SS和MDA-MB-231癌细胞系。有趣的是，所有提取物均未抑制3T3细胞的增殖，因此表明它们具有非细胞毒性特性。与氯仿提取物不同，多花野牡丹的水提取物和甲醇提取物（20、100和500μg/ml）在超氧化物清除试验中产生了高抗氧化活性，只有500μg/ml的水提取物和甲醇提取物在2,2-二苯基-1-苦基肼自由基清除试验中表现出高活性。水提取物、甲醇提取物和氯仿提取物的总酚含量分别为3344.2±19.1、3055.1±8.7和92.5±7.3mg/100g没食子酸。多花野牡丹叶具有潜在的抗增殖和抗氧化活性，这可能归因于其高含量的酚类化合物。
