Wijewardana T G, Sutherland A D
Moredun Research Institute, Edinburgh, Great Britain.
Vet Microbiol. 1990 Jul;24(1):55-62. doi: 10.1016/0378-1135(90)90050-6.
The susceptibility of Pasteurella multocida to killing by serum and the ability of protective vaccines to stimulate this mechanism of immunity in mice were investigated. P. multocida type of bovine origin was used to prepare a vaccine incorporating heat killed organisms and for homologous infection of mice. Bactericidal capacity and ELISA antibody titres were determined for individual mouse sera. Protection was clearly associated with bactericidal antibodies raised by vaccination. The bactericidal assay may be useful as a rapid, simple screening test of vaccinated mice for functional protective antibody levels.
研究了多杀性巴氏杆菌对血清杀伤的敏感性以及保护性疫苗刺激小鼠这种免疫机制的能力。使用牛源多杀性巴氏杆菌制备包含热灭活菌的疫苗，并用于小鼠的同源感染。测定了各小鼠血清的杀菌能力和ELISA抗体滴度。保护作用明显与疫苗接种产生的杀菌抗体相关。杀菌试验可作为一种快速、简单的筛选试验，用于检测接种疫苗小鼠的功能性保护性抗体水平。
