The First Affiliated Hospital of Nanjing Medical University, Nanjing, People's Republic of China.
Reprod Biomed Online. 2012 Feb;24(2):186-90. doi: 10.1016/j.rbmo.2011.11.002. Epub 2011 Nov 11.
When infertile women undergoing IVF or intracytoplasmic sperm injection (ICSI) have more than 20 mature oocytes retrieved, at least 15 oocytes are inseminated by their husband's spermatozoa. The extra oocytes are cryopreserved by vitrification. If the patients became pregnant and have healthy live births, the patients are encouraged to donate their remaining cryopreserved oocytes. Forty-seven egg-sharing donors were recruited after having normal deliveries and they donated their remaining oocytes, totalling 395 cryopreserved oocytes, to 75 recipients. The survival rate of vitrified-warmed oocytes was 83.0%. Following insemination by ICSI, the fertilization and cleavage rates were 83.8% and 89.8%, respectively. Out of 75 recipients, 71 recipients completed the treatment cycles and 30 of them became pregnant with clinical pregnancy and implantation rates of 42.3% and 25.5%, respectively. The birthweight of the new-born infants (22 from singleton and two from one set of twins) were 3344.5 ± 669.1g and 2425.0 ± 742.5 g, respectively. No birth defects were observed for the live births. These results indicate that oocyte vitrification is an effective methodology for an egg-sharing donation programme, with acceptable pregnancy and implantation rates.
当接受体外受精或胞浆内单精子注射 (ICSI) 的不孕女性取回 20 多个成熟卵母细胞时，至少有 15 个卵母细胞用其丈夫的精子进行授精。多余的卵母细胞通过玻璃化冷冻保存。如果患者怀孕并产下健康的活产婴儿，鼓励患者捐献其剩余的冷冻保存卵母细胞。75 名接受者共接受了 47 名正常分娩后的卵母细胞捐献者捐献的 395 个冷冻保存卵母细胞。玻璃化冷冻解冻后的卵母细胞存活率为 83.0%。经 ICSI 授精后，受精率和卵裂率分别为 83.8%和 89.8%。在 75 名接受者中，有 71 名完成了治疗周期，其中 30 名怀孕，临床妊娠率和着床率分别为 42.3%和 25.5%。新生儿（22 名单胎和 2 名双胞胎）的出生体重分别为 3344.5 ± 669.1g 和 2425.0 ± 742.5 g。活产儿无出生缺陷。这些结果表明，卵母细胞玻璃化是一种有效的卵子共享捐赠计划方法，具有可接受的妊娠和着床率。
