State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China.
Bioresour Technol. 2012 Feb;106:36-43. doi: 10.1016/j.biortech.2011.11.112. Epub 2011 Dec 3.
The effect of solids retention time on reactor performance and microbial community composition in anoxic/aerobic membrane bioreactors (A/O-MBR) were investigated in this study. Experimental results showed high removal efficiencies of conventional pollutants. Antibiotics removal efficiencies were obviously affected by SRT changes. Longer SRT (above 30days) was proved to be suitable operational condition for antibiotics removal. Denaturing gradient gel electrophoresis (DGGE) and clone library analysis revealed that bacteria belong to Betaproteobacteria and Gammaproteobacteria were the dominant species during wastewater treatment and antibiotics removal. SRT significantly influenced the relative numbers of nitrifying bacteria. Removal efficiency of total nitrogen (TN) decreased when SRT was 3days, because nitrogen loading exceeded the denitrification ability of the reactors. Unlike tet C and tet E genes, sulfa antibiotics resistance gene presented a decreasing tendency with the decrease of SRT, and finally affected sulfa antibiotics removal efficiencies.
本研究考察了固体停留时间（SRT）对缺氧/好氧膜生物反应器（A/O-MBR）中反应器性能和微生物群落组成的影响。实验结果表明，常规污染物的去除效率较高。抗生素的去除效率明显受到 SRT 变化的影响。较长的 SRT（超过 30 天）被证明是抗生素去除的合适操作条件。变性梯度凝胶电泳（DGGE）和克隆文库分析表明，在废水处理和抗生素去除过程中，细菌属于β变形菌纲和γ变形菌纲。SRT 显著影响硝化细菌的相对数量。当 SRT 为 3 天时，总氮（TN）的去除效率降低，因为氮负荷超过了反应器的反硝化能力。与 tet C 和 tet E 基因不同，磺胺类抗生素抗性基因随着 SRT 的降低呈下降趋势，最终影响磺胺类抗生素的去除效率。
