Kidd A H, Erasmus M J, Tiemessen C T
Department of Virology, Auckland Hospital, New Zealand.
Virology. 1990 Nov;179(1):139-50. doi: 10.1016/0042-6822(90)90283-w.
The fiber gene of adenovirus type 41 was sequenced and compared to the fiber gene sequence of adenovirus type 40 (A. H. Kidd and M. J. Erasmus, 1989, Virology 172, 134-144), the other known member of subgroup F. The open reading frame, from map units 87 through 92 with transcription from the r-strand, comprised 1686 bases and was 45 bases longer than its counterpart on the Ad40 genome. The 45-base difference appears to have resulted from a block deletion on the Ad40 sequence. Apart from this one region, the Ad40 and Ad41 fiber genes showed remarkably high homology (95.6%), indicating a relatively recent evolutionary divergence. The deduced amino acid sequence of the Ad41 fiber polypeptide was analyzed according to the model of N. M. Green et al. (1983, EMBO J. 2, 1357-1365) for the structure of the adenovirus fiber. Ad41 had one more 15-residue repeat in the shaft region than Ad40, there being 22 repeat motifs. A detailed study of various Ad40 and Ad41 strains with proven genome differences indicated that the 15-amino acid difference in polypeptide length at the 14th repeat motif is a type-specific difference among the subgroup F adenoviruses. However, two uncommon Ad41 strains belonging to 2 of the 16 Ad41 genome types tested had a 15-amino acid block deletion which was different to that of the Ad40 polypeptide. The implication from this work is that the Ad40 fiber gene probably arose from its Ad41 counterpart, but the fiber gene sequences of both types of subgroup F adenovirus are so similar that genetic recombination between strains could occur with some frequency.
对腺病毒41型的纤维基因进行了测序，并与腺病毒40型（A.H.基德和M.J.伊拉斯谟，1989年，《病毒学》172卷，134 - 144页）的纤维基因序列进行了比较，腺病毒40型是F亚组的另一个已知成员。从图距单位87到92的开放阅读框，以r链转录，由1686个碱基组成，比腺病毒40型基因组上的对应序列长45个碱基。这45个碱基的差异似乎是由腺病毒40型序列上的一个片段缺失导致的。除了这一区域外，腺病毒40型和41型的纤维基因显示出非常高的同源性（95.6%），表明它们在进化上的分歧相对较近。根据N.M.格林等人（1983年，《欧洲分子生物学组织杂志》2卷，1357 - 1365页）关于腺病毒纤维结构的模型，对腺病毒41型纤维多肽的推导氨基酸序列进行了分析。腺病毒41型在杆状区域比腺病毒40型多一个15个残基的重复序列，共有22个重复基序。对各种已证实基因组存在差异的腺病毒40型和41型毒株的详细研究表明，第14个重复基序处多肽长度的15个氨基酸差异是F亚组腺病毒之间的型特异性差异。然而，在测试的16种腺病毒41型基因组类型中的2种中，两种不常见的腺病毒41型毒株有一个15个氨基酸的片段缺失，这与腺病毒40型多肽的缺失不同。这项工作的意义在于，腺病毒40型纤维基因可能起源于其腺病毒41型对应基因，但两种F亚组腺病毒的纤维基因序列非常相似，以至于毒株之间可能会频繁发生基因重组。
