School of Life Science, Southwest University, Chongqing 400715, China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2012 Jan 15;881-882:49-54. doi: 10.1016/j.jchromb.2011.11.040. Epub 2011 Dec 3.
It is known that the choice of solvent system for high speed counter-current chromatography separation is of utmost importance. In this study, a simple and rapid thin layer chromatograph coupling with fluorometric (TLC-F) method has been used to determine the partition coefficient of target compounds in HSCCC solvent system. Two components, 6,7-dimethoxycoumarin and 5-hydroxymethyl-2-furfural were successfully separated from purple sweet potato extracts by successive sample injection for the first time, using n-hexane-ethyl acetate-methanol-water (1:2:1:1, v/v/v/v) as the solvent system. Additionally, statistical analysis showed that there was no significant difference in partition coefficient obtained by the TLC-F method and by HPLC, which demonstrated the usefulness of TLC-F method.
已知溶剂系统的选择对于高速逆流色谱分离至关重要。在本研究中，采用简单快速的薄层色谱-荧光（TLC-F）法测定目标化合物在 HSCCC 溶剂系统中的分配系数。首次成功地通过连续进样，从紫薯提取物中分离出两种成分，6,7-二甲氧基香豆素和 5-羟甲基-2-糠醛，使用正己烷-乙酸乙酯-甲醇-水（1:2:1:1，v/v/v/v）作为溶剂系统。此外，统计分析表明，TLC-F 法和 HPLC 法得到的分配系数无显著差异，这证明了 TLC-F 法的有用性。
