Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
Gene. 2012 Feb 15;494(1):1-10. doi: 10.1016/j.gene.2011.12.015. Epub 2011 Dec 16.
Haruan (Channa striatus) is in great demand in the Malaysian domestic fish market. In the present study, mtDNA cyt b was used to investigate genetic variation of C. striatus among populations in Peninsular Malaysia. The overall population of C. striatus demonstrated a high level of haplotype diversity (h) and a low-to-moderate level of nucleotide diversity (π). Analysis of molecular variance (AMOVA) results showed a significantly different genetic differentiation among 6 populations (F(ST)=0.37566, P=0.01). Gene flow (Nm) was high and ranged from 0.32469 to infinity (∞). No significant relationship between genetic distance and geographic distance was detected. A UPGMA tree based on the distance matrix of net interpopulation nucleotide divergence (d(A)) and haplotype network of mtDNA cyt b revealed that C. striatus is divided into 2 major clades. The neutrality and mismatch distribution tests for all populations suggested that C. striatus in the study areas had undergone population expansion. The estimated time of population expansion in the mtDNA cyt b of C. striatus populations occurred 0.72-6.19 million years ago. Genetic diversity of mtDNA cyt b and population structure among Haruan populations in Peninsular Malaysia will be useful in fisheries management for standardization for Good Agriculture Practices (GAP) in fish-farming technology, as well as providing the basis for Good Manufacturing Practices (GMP).
巨魾（Channa striatus）在马来西亚国内鱼类市场的需求量很大。本研究采用 mtDNA cyt b 来研究马来西亚半岛 C. striatus 种群的遗传变异。C. striatus 的总体种群表现出高单倍型多样性（h）和低至中等水平的核苷酸多样性（π）。分子方差分析（AMOVA）结果表明，6 个种群之间存在显著的遗传分化（F(ST)=0.37566，P=0.01）。基因流动（Nm）较高，范围从 0.32469 到无穷大（∞）。遗传距离与地理距离之间没有显著的关系。基于净种群间核苷酸分歧（d(A)）的距离矩阵和 mtDNA cyt b 的单倍型网络图构建的 UPGMA 树表明，C. striatus 分为 2 个主要分支。所有种群的中性和不匹配分布检验表明，研究区域的 C. striatus 经历了种群扩张。C. striatus 种群 mtDNA cyt b 的估计种群扩张时间发生在 0.72-6.19 百万年前。马来西亚半岛巨魾的 mtDNA cyt b 的遗传多样性和种群结构将有助于渔业管理标准化，以实现鱼类养殖技术的良好农业规范（GAP），并为良好生产规范（GMP）提供基础。
