在大鼠皮层桶状结构中经验驱动的突触可塑性过程中，AMPA 受体亚基的发育特异性。
Developmental AMPA receptor subunit specificity during experience-driven synaptic plasticity in the rat barrel cortex.
机构信息
Yokohama City University Graduate School of Medicine, Department of Physiology, 3-9 Fukuura, Kanazawa-ku, Yokohama 236-0004, Japan.
出版信息
Brain Res. 2012 Jan 30;1435:1-7. doi: 10.1016/j.brainres.2011.11.033. Epub 2011 Nov 19.
During early postnatal brain development, experience-driven delivery of AMPA receptors to synapses participates in the initial organization of cortical function. By combining virus-mediated in vivo gene delivery with in vitro whole cell recordings, we identified a subunit-specific developmental program of experience-driven AMPA receptor delivery to synapses in rat barrel cortex. We expressed green fluorescent protein (GFP)-tagged AMPA receptors (GFP-GluR1, or GFP-GluR4) into layer 2/3 pyramidal neurons at two distinct developmental periods, postnatal day (P)8-P10 and P12-P14. Two days after viral infection, acute brain slices were prepared, and synaptic transmission from layer 4 to layer 2/3 was analyzed by whole cell recordings. We found that whisker experience drives GluR4 but not GluR1 into these synapses early in postnatal development (P8-P10). However, at P12-14, GluR1 but not GluR4 is delivered into synapses by whisker experience. This precise developmental plan suggests unique plasticity properties endowed in different AMPA receptor subunits which shape the initial experience-driven organization of cortical function.
在早期的产后大脑发育过程中，AMPA 受体受经验驱动而向突触传递，参与了皮质功能的初步组织。通过将病毒介导的体内基因传递与体外全细胞记录相结合，我们在大鼠桶状皮层中确定了一个与经验驱动的 AMPA 受体向突触传递有关的亚基特异性发育程序。我们在两个不同的发育阶段（P8-P10 和 P12-P14）将 GFP 标记的 AMPA 受体（GFP-GluR1 或 GFP-GluR4）表达到第 2/3 层的锥体神经元中。病毒感染两天后，制备急性脑切片，并通过全细胞记录分析来自第 4 层到第 2/3 层的突触传递。我们发现，在产后早期发育（P8-P10）期间，胡须经验将 GluR4 而不是 GluR1 驱动到这些突触中。然而，在 P12-14 时，胡须经验将 GluR1 而不是 GluR4 传递到突触中。这种精确的发育计划表明，不同的 AMPA 受体亚基具有独特的可塑性，这些可塑性赋予了皮质功能的初始经验驱动组织。
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