Division of Clinical Chemistry, Department of Laboratory Medicine, Lund University, Skåne University Hospital, Malmö, Sweden.
Nanomedicine. 2012 Aug;8(6):981-6. doi: 10.1016/j.nano.2011.12.001. Epub 2011 Dec 23.
The association of nanoparticles (NPs) with blood coagulation proteins may influence the natural balance between pro- and anticoagulant pathways. We investigated whether polystyrene NPs, when added to human plasma, affected the generation of thrombin in plasma. Amine-modified NPs were found to decrease the thrombin formation due to binding of factors VII and IX to the NPs, which resulted in depletion of the respective protein in solution. In contrast, carboxyl-modified NPs were able to act as a surface for activation of the intrinsic pathway of blood coagulation in plasma. These results highlight the influence of NPs on a biologically important pathway.
纳米粒子（NPs）与血液凝血蛋白的结合可能会影响促凝和抗凝途径之间的自然平衡。我们研究了聚苯乙烯 NPs 加入人血浆后是否会影响血浆中凝血酶的生成。研究发现，由于因子 VII 和 IX 与 NPs 的结合，胺修饰的 NPs 减少了凝血酶的形成，从而导致相应蛋白质在溶液中的耗竭。相比之下，羧基修饰的 NPs 能够作为血浆中内源性凝血途径激活的表面。这些结果强调了 NPs 对生物重要途径的影响。
