Adult Stem Cell Research Center, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea.
Prev Med. 2012 May;54 Suppl:S42-9. doi: 10.1016/j.ypmed.2011.11.013. Epub 2011 Dec 8.
Stem cells have been considered for their potential in pharmaceutical research, as well as for stem cell-based therapy for many diseases. Despite the potential for their use, the challenge remains to examine the safety and efficacy of stem cells for their use in therapies. Recently, oxidative stress has been strongly implicated in the functional regulation of cell behavior of stem cells. Therefore, development of rapid and sensitive biomarkers, related to oxidative stress is of growing importance in stem cell-based therapies for treating various diseases. Since stem cells have been implicated as targets for carcinogenesis and might be the origin of "cancer stem cells", understanding of how oxidative stress-induced signaling, known to be involved in the carcinogenic process could lead to potential screening of cancer chemopreventive and chemotherapeutic agents. An evaluation of antioxidant states reducing equivalents like GSH and superoxide dismutase (SOD), as well as reactive oxygen species (ROS) and nitric oxide (NO) generation, can be effective markers in stem cell-based therapies. In addition, oxidative adducts, such as 4-hydroxynonenal, can be reliable markers to detect cellular changes during self-renewal and differentiation of stem cells. This review highlights the biomarker development to monitor oxidative stress response for stem cell-based chemical screening.
干细胞因其在药物研究中的潜在应用以及基于干细胞的多种疾病治疗而备受关注。尽管具有应用潜力，但仍面临挑战，需要研究干细胞在治疗中的安全性和有效性。最近，氧化应激强烈影响干细胞的细胞行为的功能调节。因此，开发与氧化应激相关的快速而敏感的生物标志物对于基于干细胞的治疗各种疾病变得越来越重要。由于干细胞被认为是致癌作用的靶点，并且可能是“癌症干细胞”的起源，因此了解氧化应激诱导的信号转导如何参与致癌过程，可能会导致潜在的癌症化学预防和化学治疗药物的筛选。评估抗氧化状态（如 GSH 和超氧化物歧化酶（SOD））以及活性氧（ROS）和一氧化氮（NO）的产生，可以作为基于干细胞的治疗中的有效标志物。此外，氧化加合物，如 4-羟基壬烯醛，可以作为可靠的标志物来检测干细胞自我更新和分化过程中的细胞变化。本综述强调了生物标志物的开发，以监测基于干细胞的化学筛选中的氧化应激反应。
