Schimming Tobias T, Grabellus Florian, Roner Mathias, Pechlivanis Sonali, Sucker Antje, Bielefeld Nicola, Moll Iris, Schadendorf Dirk, Hillen Uwe
Department of Dermatology, Venerology and Allergology, University Duisburg-Essen, Germany.
Am J Dermatopathol. 2012 May;34(3):266-9. doi: 10.1097/DAD.0b013e31823135a3.
According to the seventh edition of the American Joint Committee on Cancer guidelines, the TNM staging category in thin cutaneous melanomas depends on the mitotic rate (MR). In this study, we analyze the interobserver agreement of the MR in a series of 92 thin cutaneous melanomas. Serial sections of the tumors were either stained with hematoxylin and eosin or immunohistochemically stained with pHH3, an antibody for phosphohistone H3, and analyzed by 4 observers. Determination of MR with pHH3 immunostaining resulted in higher sensitivity in counting mitosis for all observers. Moreover, interobserver agreement was higher with pHH3. Immunostaining with pHH3 is a sensitive method to detect mitosis in thin cutaneous melanomas, with good reproducibility of MR between independent observers. Further studies are needed to find out if higher sensitivity in the detection of mitosis by pHH3 immunostaining has additional prognostic relevance.
根据美国癌症联合委员会第七版指南，薄型皮肤黑色素瘤的TNM分期类别取决于有丝分裂率（MR）。在本研究中，我们分析了92例薄型皮肤黑色素瘤系列中MR的观察者间一致性。肿瘤的连续切片要么用苏木精和伊红染色，要么用pHH3（一种磷酸化组蛋白H3抗体）进行免疫组化染色，并由4名观察者进行分析。用pHH3免疫染色测定MR对所有观察者而言，在计数有丝分裂时具有更高的敏感性。此外，观察者间用pHH3的一致性更高。用pHH3免疫染色是检测薄型皮肤黑色素瘤中有丝分裂的一种敏感方法，独立观察者之间MR的重现性良好。需要进一步研究以确定通过pHH3免疫染色在检测有丝分裂方面的更高敏感性是否具有额外的预后相关性。
