Department of Oncology, Xiangya Hospital, Central South University, Changsha, Hunan Province 410008, PR China.
Oncol Rep. 2012 Mar;27(3):791-7. doi: 10.3892/or.2011.1603. Epub 2011 Dec 21.
Magnetic stent hyperthermia (MSH) is a novel approach for targeted thermotherapy for esophageal cancer, which is based on the mechanism that inductive heat can be generated by the esophageal stent upon exposure under an alternative magnetic field (AMF). A positive effect of MSH on esophageal cancer has been demonstrated, however, there is no study on the in vitro effects of heating treatment or of the effects of AMF exposure on human esophageal cancer cells. This study aimed to investigate the effect of MSH and of AMF exposure in esophageal cancer cells. Inductive heating characteristics of esophageal stents were assessed by exposing the stents under AMF. A rather rapid temperature rise of the Ni-Ti stent when subjected to AMF exposure was observed and the desired hyperthermic temperature could be controlled by adjusting the field parameter of the AMF. Human esophageal squamous carcinoma (ESCC) ECA-109 cells were divided into four groups: the control group, the water-bath heating group, the MSH group and the AMF exposure group. Hyperthermic temperatures were 43, 48 and 53˚C and the treatment time was in the range of 5-30 min. The MTT assay, apoptotic analysis and TUNEL staining were applied in the current investigation. Exposure of ECA-109 cells under AMF with a field intensity of 50 to 110 kA/m had negligible effect on cell viability, cell necrosis and apoptosis. Hyperthermia had a remarkable inhibitory effect on the cell viability and the effect was dependent on the thermal dose (temperature and time). The optimal thermal dose of MSH for ECA-109 cells was 48˚C for 20-30 min. The study also elucidated that there was a difference in the effects on cell necrosis and apoptosis between the heating mode of water bath and MSH. The data suggest that MSH may have clinical significance for esophageal cancer treatment.
磁控支架热疗（MSH）是一种针对食管癌的靶向热疗新方法，其基于在交变磁场（AMF）下，食管支架会产生感应热的机制。MSH 对食管癌有积极影响，但目前尚无关于加热处理的体外效应或 AMF 暴露对人食管癌细胞影响的研究。本研究旨在探讨 MSH 和 AMF 暴露对食管癌细胞的影响。通过将支架暴露在 AMF 下来评估食管支架的感应加热特性。观察到 Ni-Ti 支架在受到 AMF 暴露时会迅速升温，并且可以通过调整 AMF 的场参数来控制所需的热疗温度。将人食管鳞状细胞癌（ESCC）ECA-109 细胞分为四组：对照组、水浴加热组、MSH 组和 AMF 暴露组。热疗温度分别为 43、48 和 53℃，处理时间为 5-30 min。本研究应用 MTT 检测法、凋亡分析和 TUNEL 染色法。将 ECA-109 细胞暴露于场强为 50-110 kA/m 的 AMF 下，对细胞活力、细胞坏死和凋亡几乎没有影响。热疗对细胞活力有显著抑制作用，其效果取决于热剂量（温度和时间）。MSH 对 ECA-109 细胞的最佳热剂量为 48℃，处理时间为 20-30 min。研究还阐明了水浴和 MSH 加热方式对细胞坏死和凋亡的影响存在差异。数据表明，MSH 可能对食管癌的治疗具有临床意义。
