Yamaguchi Mizuka, Kakimoto Kensaku, Yamaguchi Takahiro, Obana Hirotaka
Osaka Prefectural Institute of Public Health, Japan.
Shokuhin Eiseigaku Zasshi. 2011;52(5):281-6. doi: 10.3358/shokueishi.52.281.
A method using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) was developed for the simultaneous determination of polyether antibiotics and macrolide anthelmintics in livestock products. The polyether antibiotics and macrolide anthelmintics were extracted from livestock products with acetonitrile and cleaned up with dispersive solid-phase extractions and a silica gel column. The quantification limits of polyether antibiotics and macrolide anthelmintics were 0.00005-0.0005 µg/g. Except for narasin and lasalocid in bovine liver and milk, the recoveries were 70 to 117%. The relative standard deviations met the required guideline. The developed method was applied to six kinds of livestock products.
建立了一种液相色谱-串联质谱联用（LC-MS/MS）方法，用于同时测定畜产品中的聚醚类抗生素和大环内酯类驱虫药。聚醚类抗生素和大环内酯类驱虫药采用乙腈从畜产品中提取，经分散固相萃取和硅胶柱净化。聚醚类抗生素和大环内酯类驱虫药的定量限为0.00005-0.0005μg/g。除牛肝和牛奶中的那拉菌素和拉沙洛西外，回收率为70%至117%。相对标准偏差符合要求的准则。所建立的方法应用于六种畜产品。
