Kondo Takahide, Kaburagi Yasuo, Shibata Masahisa, Kurokawa Chieko, Inoue Yutaka, Yamamoto Yoshihiko, Miyazaki Motonobu
Division of Life Science, Saitama City Institute of Health Science and Research, Saitama 338–0013, Japan.
Shokuhin Eiseigaku Zasshi. 2012;53(2):75-84. doi: 10.3358/shokueishi.53.75.
A simple clean-up method was developed for the simultaneous determination of pesticide residues in livestock products by GC-MS/MS. The pesticide residues were extracted with acetonitrile-ethanol (1 : 1), and matrix components such as adipose were effectively eliminated by a combination of refrigerated centrifugation, dispersive solid-phase extraction, and multifunctional column chromatography. In this method, samples are treated quickly and easily without the need for gel-permeation chromatography. Among 131 pesticides tested, 115 showed recovery within the range from 70 to 120%, with relative standard deviations of less than 15%. The quantification limits for the 115 pesticides in livestock products were 0.001 to 0.01 µg/g.
建立了一种简单的净化方法，用于气相色谱-串联质谱法同时测定畜产品中的农药残留。农药残留用乙腈-乙醇（1:1）提取，通过冷冻离心、分散固相萃取和多功能柱色谱相结合的方法有效去除脂肪等基质成分。该方法无需凝胶渗透色谱，样品处理快速简便。在测试的131种农药中，115种的回收率在70%至120%之间，相对标准偏差小于15%。这115种农药在畜产品中的定量限为0.001至0.01 µg/g。
