[基于液相色谱-串联质谱法的加工谷物类食品和奶粉中蜡样芽胞杆菌毒素的分析方法]
[Analytical method of cereulide in processed cereal-based foods and milk powder by LC-MS/MS].
作者信息
Yatsukawa Yoh-ichi, Iida Hanako, Nagata Kazuko, Miyamoto Masashi, Matsuda Takahiro, Ito Hironobu, Nakamura Munetomo, Fujita Kazuhiro
机构信息
Saito Laboratory, Japan Food Research Laboratories, Ibaraki-shi, Osaka, Japan.
出版信息
Shokuhin Eiseigaku Zasshi. 2011;52(5):287-93. doi: 10.3358/shokueishi.52.287.
An LC-MS/MS method for analysis of cereulide, an emetic toxin produced by Bacillus cereus, was developed. Cereulide was extracted from samples, fried rice, pan-fried noodles, red bean paste and baby formula, with methanol and purified using Oasis HLB cartridges. LC separation was performed on a C18 column with a mixture of formic acid solution and methanol containing ammonium formate as a mobile phase, and the mass spectrometer was operated in the positive electrospray ionization mode. Performance evaluation showed that trueness was higher than 70% and repeatability and reproducibility were within 10%. The limits of quantification were lower than 1 µg/kg.
开发了一种用于分析蜡样芽孢杆菌产生的催吐毒素cereulide的液相色谱-串联质谱（LC-MS/MS）方法。从样品（炒饭、炒面、红豆沙和婴儿配方奶粉）中用甲醇提取cereulide，并使用Oasis HLB柱进行纯化。在C18柱上进行LC分离，以含有甲酸铵的甲酸溶液和甲醇的混合物作为流动相，质谱仪在正电喷雾电离模式下运行。性能评估表明，准确性高于70%，重复性和再现性在10%以内。定量限低于1 µg/kg。
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