State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan 410082, People's Republic of China.
Org Lett. 2012 Jan 20;14(2):432-5. doi: 10.1021/ol202706k. Epub 2011 Dec 27.
Coumarin-rhodamine-based compounds 1a,b were rationally designed and synthesized as novel FRET ratiometric fluorescent chemodosimeters. Ratiometric chemodosimeters 1a,b exhibit several favorable features, including a large variation in the emission ratio, well-resolved emission peaks, high sensitivity, high selectivity, low cytotoxicity, and good cell membrane permeability. Importantly, these excellent attributes enable us to demonstrate ratiometric imaging of Cu(2+) in living cells by using these novel ratiometric fluorescent chemodosimeters.
香豆素-罗丹明类化合物 1a,b 被合理设计和合成，作为新型的 FRET 比率型荧光化学传感器。比率型化学传感器 1a,b 具有几个有利的特点，包括发射比的大变化、分辨良好的发射峰、高灵敏度、高选择性、低细胞毒性和良好的细胞膜通透性。重要的是，这些优良的特性使我们能够利用这些新型比率型荧光化学传感器在活细胞中实现 Cu(2+)的比率成像。
