Kondo Takahito, Suzuki Mamoru, Konomi Ujimoto, Otsuka Koji, Inagaki Taro, Shimizu Shigetaka, Ogawa Yasuo
Department of Otorhinolaryngology, Tokyo Medical University, Japan.
Acta Otolaryngol. 2012 Mar;132(3):228-33. doi: 10.3109/00016489.2011.635385. Epub 2011 Dec 27.
Various changes were observed in the cupula, including shrinkage and enlarged volume, following the disruption of the membranous labyrinth. Cupular change after membranous labyrinth disruption may be a pathology of vestibular disorders.
To observe the morphological changes of the cupula after disruption of the membranous labyrinth and to compare the cupular changes with changes in the compound action potential (CAP) of the ampullary nerve.
A labyrinthine injury model was created by puncturing the membranous labyrinth of bullfrogs. The cupula was observed from 3 to 17 days after the membrane puncture. The CAP in response to mechanical endolymphatic flow was recorded from the ampullary nerve. The correlation between cupular change and CAP positivity was evaluated using the authors' scale.
Various kinds of cupular changes including shrinkage were observed. Cupular change was more severe after a longer survival period. Large or elongated volume of the cupula was also observed, which was not observed in our previous study using gentamicin. The CAP could be recorded even when the cupular change was severe.
膜迷路破坏后，壶腹嵴出现了包括萎缩和体积增大等多种变化。膜迷路破坏后的壶腹嵴变化可能是前庭疾病的一种病理表现。
观察膜迷路破坏后壶腹嵴的形态学变化，并将壶腹嵴的变化与壶腹神经复合动作电位（CAP）的变化进行比较。
通过穿刺牛蛙的膜迷路建立迷路损伤模型。在膜穿刺后3至17天观察壶腹嵴。记录壶腹神经对机械性内淋巴流动的CAP。使用作者的评分标准评估壶腹嵴变化与CAP阳性之间的相关性。
观察到包括萎缩在内的各种壶腹嵴变化。存活时间较长后，壶腹嵴变化更严重。还观察到壶腹嵴体积增大或拉长，这在我们之前使用庆大霉素的研究中未观察到。即使壶腹嵴变化严重，也可记录到CAP。
