Rajaei Samira, Zarnani Amir Hassan, Jeddi-Tehrani Mahmood, Tavakoli Maryam, Mohammadzadeh Afsaneh, Dabbagh Ali, Mirahmadian Mahroo
Department of Immunology, Faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran, e-mail:
Iran J Immunol. 2011 Dec;8(4):201-8.
Repeated Implantation Failure (RIF) is one of the most intricate obstacles in assisted reproduction. The cytokine and chemokine composition of uterine cavity seem to play important roles in the implantation process.
To compare the cytokine profile in the endometrium of normal fertile women and those with repeated implantation failure.
After enzymatic digestion of endometrial tissues, whole endometrial cells and endometrial stromal cells from RIF and normal fertile women were cultivated and stimulated for cytokine secretion. The levels of IL-10, TGF-β, IFN-γ, IL-6, IL-8 and IL-17 in culture supernatants of the two groups were assayed by ELISA and compared together.
Endometrial stromal cells and whole endometrial cells of normal fertile women produced higher levels of IL-6, IL-8 and TGF-β compared to RIF group, although this difference was statistically significant only in endometrial stromal cells (p=0.005, 0.002 and 0.001, respectively). In addition, endometrial stromal cells of normal fertile women produced lower levels of IL-10 in comparison with RIF group (p=0.005).
Disturbances in cytokine production at the feto-maternal interface could be a cause of implantation failure. A pro-inflammatory cytokine milieu seems to be pivotal for successful implantation.
反复种植失败（RIF）是辅助生殖中最复杂的障碍之一。子宫腔的细胞因子和趋化因子组成似乎在着床过程中起重要作用。
比较正常生育妇女和反复种植失败妇女子宫内膜中的细胞因子谱。
对子宫内膜组织进行酶消化后，培养来自反复种植失败妇女和正常生育妇女的全子宫内膜细胞和子宫内膜基质细胞，并刺激其分泌细胞因子。采用酶联免疫吸附测定法（ELISA）检测两组培养上清液中白细胞介素-10（IL-10）、转化生长因子-β（TGF-β）、干扰素-γ（IFN-γ）、白细胞介素-6（IL-6）、白细胞介素-8（IL-8）和白细胞介素-17（IL-17）的水平，并进行比较。
与反复种植失败组相比，正常生育妇女的子宫内膜基质细胞和全子宫内膜细胞产生的IL-6、IL-8和TGF-β水平更高，尽管这种差异仅在子宫内膜基质细胞中具有统计学意义（分别为p=0.005、0.002和0.001）。此外，与反复种植失败组相比，正常生育妇女的子宫内膜基质细胞产生的IL-10水平较低（p=0.005）。
母胎界面细胞因子产生的紊乱可能是种植失败的原因。促炎细胞因子环境似乎对成功着床至关重要。
