Biomedical Research Unit, Jessop Wing, Tree Root Walk, Sheffield S10 2SF, UK.
J Reprod Immunol. 2010 Dec;87(1-2):60-6. doi: 10.1016/j.jri.2010.07.001. Epub 2010 Aug 30.
Several studies have suggested that endometrial uNK (CD56+) cells may play a role in implantation. The aim of this study was to investigate the number of CD56+, CD16+ and CD69+ cells in the unstimulated endometrium of women with recurrent implantation failure after IVF. The percentage of stromal cells positive for CD56, CD16 and CD69 was identified by immunocytochemistry in endometrial biopsies from 15 normal control women and 40 women with recurrent implantation failure. All biopsies were obtained on days LH+7 to LH+9. The density of CD56+ cells in endometrium from women with repeated implantation failure after IVF [median (range) CD56+ cell density=14.5% (1.5-71.4%)] was significantly higher (P=0.005) than in endometrium from control women [5% (2.1-19.2%)]. There was no significant difference in the densities of CD16+ and CD69+ cells in the endometrium from women in the two groups. The increased density of CD56+ cells in the endometrium of women with recurrent implantation failure suggests that these cells are directly involved in the implantation process; alternatively this may indicate a general endometrial defect in these women, which leads to the inability of the embryo to implant.
几项研究表明，子宫内膜自然杀伤（uNK）细胞（CD56+）可能在着床中发挥作用。本研究旨在探讨反复体外受精（IVF）着床失败患者的未刺激子宫内膜中 CD56+、CD16+和 CD69+细胞的数量。通过免疫细胞化学鉴定来自 15 名正常对照组和 40 名反复 IVF 着床失败患者的子宫内膜活检中 CD56、CD16 和 CD69 阳性的基质细胞的百分比。所有活检均在 LH+7 至 LH+9 天进行。反复 IVF 着床失败患者的子宫内膜中 CD56+细胞的密度[中位数（范围）CD56+细胞密度=14.5%（1.5-71.4%）]明显高于对照组[5%（2.1-19.2%）]（P=0.005）。两组患者子宫内膜中 CD16+和 CD69+细胞的密度无显著差异。反复 IVF 着床失败患者子宫内膜中 CD56+细胞密度增加表明这些细胞直接参与着床过程；或者这可能表明这些女性存在一般的子宫内膜缺陷，导致胚胎无法着床。
