Baselga R, Amorena B
EEAD (CSIC), Dept. of Animal Production, SIA (DGA), Zaragoza, Spain.
Zentralbl Veterinarmed B. 1990 Sep;37(7):556-60. doi: 10.1111/j.1439-0450.1990.tb01096.x.
A total of 59 mastitis staphylococcic strains were tested for growth agglutination upon supplementation of growth media with ovine and bovine milk whey and mammary secretions from dry cows. Differences were observed when comparing bacterial species or origins (ovine vs. bovine) of bacteria and whey. All of the ovine and bovine S. aureus strains tested, but only 4 among 22 other ovine mastitis staphylococcic strains, showed growth agglutination in Todd Hewitt broth (THB) supplemented with greater than or equal to 30% (v/v) ovine milk whey. None of the strains agglutinated during growth in regular THB medium. Ovine whey had an agglutination induction capacity higher than bovine whey (P less than 0.005), concerning the number of responsive ovine and bovine S. aureus strains. There were no differences between whey samples from different ewes with regard to their capacity to induce agglutination. Ovine S. aureus strains were more responsive than bovine strains of this bacterial species, concerning the number of responsive strains (P less than 0.001) to bovine whey (greater than or equal to 30% in THB), the proportion of responsive strains at low (10%) ovine whey concentration (P less than 0.001), and the strength of reaction (precipitation timing and clump size). Secretions from dry cows systematically induced agglutination in all of the bovine and ovine S. aureus strains tested.
总共对59株乳腺炎葡萄球菌菌株进行了测试，检测在生长培养基中添加绵羊奶和牛奶乳清以及干奶牛乳腺分泌物后它们的生长凝集情况。在比较细菌种类或细菌及乳清的来源（绵羊与牛）时观察到了差异。所有测试的绵羊和牛源金黄色葡萄球菌菌株，但在其他22株绵羊乳腺炎葡萄球菌菌株中只有4株，在添加了大于或等于30%（v/v）绵羊奶乳清的托德-休伊特肉汤（THB）中表现出生长凝集。在常规THB培养基中生长时，没有菌株发生凝集。就有反应的绵羊和牛源金黄色葡萄球菌菌株数量而言，绵羊乳清的凝集诱导能力高于牛乳清（P<0.005）。不同母羊的乳清样品在诱导凝集的能力方面没有差异。就对牛乳清（在THB中大于或等于30%）有反应的菌株数量、在低浓度（10%）绵羊乳清时反应菌株的比例（P<0.001）以及反应强度（沉淀时间和团块大小）而言，绵羊源金黄色葡萄球菌菌株比该细菌种类的牛源菌株更易产生反应。干奶牛的分泌物在所有测试的牛源和绵羊源金黄色葡萄球菌菌株中都能系统性地诱导凝集。
