通过在乳清中生长诱导来自牛乳腺炎的金黄色葡萄球菌的抗吞噬表面特性。
Induction of anti-phagocytic surface properties of Staphylococcus aureus from bovine mastitis by growth in milk whey.
作者信息
Mamo W, Sandgren C H, Lindahl M, Jonsson P
机构信息
Department of Veterinary Microbiology, Swedish University of Agricultural Sciences, Uppsala.
出版信息
Zentralbl Veterinarmed B. 1991 Aug;38(6):401-10. doi: 10.1111/j.1439-0450.1991.tb00889.x.
The respiratory burst activity of bovine polymorphonuclear (PMN) cells in response to milk whey- and TSB-grown S. aureus strains isolated from bovine mastitis was studied in whole blood chemiluminescence (CL) and in a CL system with purified bovine neutrophils. In both cases milk whey-grown S. aureus strains elicited significantly less CL than homologous strains grown in TSB. Ingestion of milk whey-grown S. aureus strains by bovine neutrophils was also considerably lower than that of the corresponding homologous organisms grown in TSB. Binding of complement factor C3 to serum-opsonized milk whey-grown S. aureus strains was lower compared with TSB-grown homologous organisms. Moreover, 5 of 6 S. aureus strains grown in milk whey were significantly more resistant to in vivo clearance from the peritoneal cavity of mice compared with homologous bacteria grown in TSB. S. aureus strains grown in TSB exhibited hydrophobic surface properties, whereas homologous strains grown in milk whey were hydrophilic.
在全血化学发光（CL）以及纯化牛中性粒细胞的CL系统中，研究了牛多形核（PMN）细胞对从牛乳腺炎分离出的、在乳清和胰酪大豆胨肉汤（TSB）中培养的金黄色葡萄球菌菌株的呼吸爆发活性。在这两种情况下，在乳清中培养的金黄色葡萄球菌菌株引发的CL明显低于在TSB中培养的同源菌株。牛中性粒细胞对在乳清中培养的金黄色葡萄球菌菌株的摄取也明显低于在TSB中培养的相应同源菌。与在TSB中培养的同源菌相比，补体因子C3与血清调理的、在乳清中培养的金黄色葡萄球菌菌株的结合较低。此外，与在TSB中培养的同源菌相比，在乳清中培养的6株金黄色葡萄球菌中有5株对从小鼠腹腔内的体内清除具有显著更高的抗性。在TSB中培养的金黄色葡萄球菌菌株表现出疏水表面特性，而在乳清中培养的同源菌株是亲水的。
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