Harłozińska A, Rabczyński J, Bar J K, Richter R
Department of Tumor Immunology, Silesian Piast's Medical Academy, Wrocław, Poland.
Acta Cytol. 1990 Sep-Oct;34(5):657-63.
Three subpopulations of cancer cells with different morphologic features were separated by density gradient centrifugation of two ascitic fluids and one cystic fluid from one patient with ovarian clear cell carcinoma and one with endometrial clear cell carcinoma. Immunophenotypic analyses of isolated fractions using polyclonal and monoclonal antibodies against ovarian carcinoma-associated antigens revealed significant immunologic heterogeneity among the tumor cells. The identical histopathologic structures of the ovarian and endometrial clear cell carcinomas and the similar distribution and immunologic reactivity of the cell types isolated from the ascitic and cystic fluids confirmed the common histogenesis of both cancers. These findings suggest that the conventional cytologic diagnosis of clear cell carcinomas could be supplemented by immunofluorescent staining. Density gradient centrifugation appeared to be a useful method for the separation of mesothelial cells.
通过对一名卵巢透明细胞癌患者和一名子宫内膜透明细胞癌患者的两份腹水和一份囊液进行密度梯度离心，分离出具有不同形态特征的三个癌细胞亚群。使用针对卵巢癌相关抗原的多克隆和单克隆抗体对分离出的组分进行免疫表型分析，结果显示肿瘤细胞之间存在显著的免疫异质性。卵巢和子宫内膜透明细胞癌相同的组织病理学结构，以及从腹水和囊液中分离出的细胞类型相似的分布和免疫反应性，证实了这两种癌症具有共同的组织发生学。这些发现表明，免疫荧光染色可作为透明细胞癌传统细胞学诊断的补充方法。密度梯度离心似乎是分离间皮细胞的一种有用方法。
